Crystal structure of tyrosine-tRNA synthetase from Helicobacter pylori G27

The production, crystallization and 2.2 Å resolution structure of H. pylori tyrosine-tRNA synthetase are reported.

Structural analysis of Helicobacter pylori glutamate racemase in a monoclinic crystal form

The crystal structure of H. pylori MurI is reported at 1.43 Å resolution.

Crystal structure of the bifunctional enzyme phosphoribosyl isomerase A from Mycobacterium tuberculosis with bound light-controllable inhibitor

We have determined the crystal structure of an isomerase catalyzing two steps in histidine and tryptophan biosynthesis with a bound light-switchable competitive inhibitor. The work explains the mode of action of the inhibitor and describes the discovery of a hitherto unknown third phosphate-binding site of the enzyme.

Recombinant versus native botulinum neurotoxin type A: structural and pharmacodynamic comparison

For the first time, the recombinant core botulinum neurotoxin type A (BoNT/A) expressed in Escherichia coli and nicked in vitro is shown to be structurally identical to native BoNT/A from Clostridium botulinum, enabling direct activity comparison between the recombinant product and the native toxin.

Crystal structures of the N-terminal and C-terminal domains of the SARS-CoV-2 nucleocapsid protein

Crystal structures of the SARS-CoV-2 N protein N-terminal domain and C-terminal domain determined at resolutions of 1.30–1.94 Å reveal a conserved N-terminal domain fold containing a positively charged cleft encompassing residues previously implicated in RNA binding and a conserved β-hairpin-mediated C-terminal domain dimer.

X-ray structure of the human Nijmegen breakage syndrome forkhead-associated/BRCA1 C-terminal domain and computational docking of candidate radiosensitizers

Nijmegen breakage syndrome 1 (NBS1), a component of the MRE11–RAD50–NBS1 complex, plays a key role in the repair of DNA double-strand breaks. We report the first experimentally determined X-ray crystal structure of the FHA–BRCT region of human NBS1 and the results of docking simulations of candidate radiosensitizing compounds.

Structure of the cupin protein GloB from Pseudomonas aeruginosa at 1.48 Å resolution

The GloB cupin domain from an unknown biosynthetic gene cluster from P. aeruginosa shows similarities to isomerase enzymes.