Biological Crystallography goes online

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Guidelines for the deposition and release of macromolecular coordinate and experimental data

The three-dimensional structure of a Trichoderma reesei β-mannanase from glycoside hydrolase family 5

The three-dimensional structure of the catalytic core domain of a T. reesei β-mannanase belonging to glycoside hydrolase family 5 has been solved and refined at 1.5 Å resolution. The starting phases were computed solely from the anomalous scattering of a single non-isomorphous platinum complex at 1.65 Å resolution.

The 2.0 Å structure of bovine interferon-γ; assessment of the structural differences between species

The structure of bovine interferon-γ, determined at 2.0 Å resolution by multiple isomorphous replacement, is compared with the other interferon-γ structures available.

Structure of a new neurotoxin from the scorpion Buthus martensii Karsch at 1.76 Å

The structure of a new neurotoxin BmK M2 from Chinese scorpion at 1.76 Å resolution has been studied with the sequence determined crystallographically. The structure further proved the existence of a non-proline cis peptide bond in the group III α-like scorpion toxins and showed the residues possibly involved in the high toxicity of BmK M2.

1.35 and 2.07 Å resolution structures of the red abalone sperm lysin monomer and dimer reveal features involved in receptor binding

The high-resolution refinement and analysis of the monomeric and dimeric forms of red abalone sperm lysin provides the basis for a model for the interaction of lysin with its egg receptor.

RSPS version 4.0: a semi-interactive vector-search program for solving heavy-atom derivatives

A program for inspection and interpretation of the Patterson function is described. Options are available for vector-based methods to locate heavy-atom sites, for finding sets from a list of possible heavy-atom positions and for checking of potential solutions.

Real-time swelling-series method improves the accuracy of lamellar neutron-diffraction data

Neutron-diffraction data have been collected form 1,2-dioleoyl-sn-glycero-phosphocholine under conditions of increasing relative humidity at 0 and 8.06% ²H₂O. These data are used to demonstrate the increased accuracy inherent in a real-time swelling-series method.

Effects of microgravity on the crystal quality of a collagen-like polypeptide

Good quality crystals of (Pro-Pro-Gly)₁₀ were successfully grown in microgravity during the STS-95 mission.

Crystallization and preliminary X-ray diffraction studies of human epidermal growth factor

Human epidermal growth factor has been crystallized in trigonal and tetragonal space groups, and 3.0 Å resolution data have been collected for its trigonal crystals.

Crystallization and preliminary X-ray diffraction studies on the N-utilizing substance-B (NusB) from Mycobacterium tuberculosis

Crystallization and preliminary X-ray diffraction studies of N-utilizing substance-B (NusB) from M. tuberculosis are reported to 1.6 Å resolution.

Crystallization and preliminary X-ray analyses of catabolite control protein A, free and in complex with its DNA-binding site

Catabolite protein A from B. megaterium has been crystallized. The hexagonal crystals diffract to 2.55 Å resolution.

Crystallization and preliminary diffraction studies of a truncated form of a novel protease from spores of Bacillus megaterium

Crystals of a truncated form of the zymogen of the germination protease from spores of B. megaterium have been obtained by the vapor-diffusion method. The results of preliminary X-ray diffraction analysis of these crystals are discussed.

Crystallization and preliminary X-ray analysis of recombinant full-length human m-calpain

Full-length human recombinant m-calpain was crystallized in two crystal forms. A 2.3 Å data set was collected from a gold derivative by MAD techniques suitable for phase determination.

Toxoplasma gondii adenosine kinase: expression, purification, characterization, crystallization and preliminary crystallographic analysis

Recombinant adenosine kinase of T. gondii expressed in E. coli was purified, characterized and crystallized using ammonium sulfate or sodium sulfate at pH 8.5. These crystals belong to space group P2₁, with one molecule of protein in the asymmetric unit.

Crystallization and preliminary X-ray diffraction studies of cytochrome c₆ from Porphyra yezoensis

Two different crystal forms of cytochrome c₆ from the red alga P. yezoensis have been obtained. Crystals belonging to space group P4₁2₁2 or P4₃2₁2 diffract to 1.8 Å resolution and crystals belonging to space group P2₁2₁2₁ diffract to 1.6 Å resolution.

Lactate de­hydrogenase from the hyperthermophilic archaeon Methanococcus jannaschii: overexpression, crystallization and preliminary X-ray analysis

Lactate dehydrogenase from hyperthermophilic archaeon M. jannaschii has been crystallized. Its native data have been collected to 1.9 Å.

Crystallization and preliminary crystallographic studies of ribosome recycling factor from Escherichia coli

Ribosome recycling factor from E. coli has been crystallized and native data have been collected at 3.0 Å resolution using Cu Kα X-rays.

Crystallization of the NADP-dependent β-keto acyl-carrier protein reductase from Brassica napus

X-ray diffraction data have been collected from crystals of the fatty acid biosynthetic enzyme β-keto reductase from B. napus to a resolution of 2.8 Å and the crystal space group and cell parameters determined (P6₄22; a = b = 129.9, c = 93.1 Å, α = β = 90, γ = 120°). An initial molecular replacement solution has been determined using the structure of the enzyme hydroxysteroid dehydrogenase from Streptomyces hydrogenans as a search model.

Crystallization and preliminary X-ray diffraction analysis of the NAD-dependent non-phosphorylating GAPDH of the hyperthermophilic archaeon Thermoproteus tenax

Recombinant non-phosphorylating NAD⁺-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) of the hyperthermophilic crenarchaeote Thermoproteus tenax has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion technique.

Crystallization and preliminary X-ray analysis of the catalytic core of the alkylhydroperoxide reductase component AhpF from Escherichia coli

Crystals of the proteolytically truncated AhpF component of the alkyl hydroperoxide reductase from E. coli have been grown under oxidizing conditions. X-ray diffraction data, collected to 1.9 Å resolution using synchrotron radiation, are reported.

Crystallization and preliminary X-ray analysis of a bacteriophage T4 primase fragment

Attempts have been made to crystallize the primase from bacteriophage T4. A C-terminal fragment including residues 196–340 gave four crystal forms, one of which diffracts to 3.5 Å resolution.

Preliminary X-ray crystallographic analysis of a plant defence protein, the polygalacturonase-inhibiting protein from Phaseolus vulgaris

Single crystals of a plant defence protein, the polygalacturonase-inhibiting protein (PGIP-1) from P. vulgaris, have been obtained. They belong to space group P2₁ and diffract to at least 2.3 Å resolution.

Crystallization of type I chloramphenicol acetyltransferase: an approach based on the concept of ionic strength reducers

The crystallization of a type I chloramphenicol acetyltransferase using the concept of ionic strength reducers is reported.

Crystallization and preliminary crystallographic study of triple-helical DNA

Single crystals of an oligonucleotide construct containing triple helical DNA have been grown. These crystals diffract to 1.8 Å at a synchrotron X-ray beamline and contain bromuracil substitutions for thymine to permit MAD analysis.

Optimization of the critical nuclear size for protein crystallization: a note

A reason why some proteins crystallize best at supersaturation ratios of 2.5–3, rather than close to the supersolubility curve of their phase diagram, is proposed.

Experiences and expectations of a novel X-ray microsource with focusing mirror. I. Erratum

An erratum to the paper by Bloomer & Arndt (1999) Acta Cryst. D55, 1672-1680.

Crystallization of Helix pomatia agglutinin (HPA), a protein from the edible snail. Erratum

An erratum to the paper by Lisgarten et al. (1999) Acta Cryst. D55, 1903–1905.

Notes for authors