Structures of alternatively spliced isoforms of human ketohexokinase

The structures of the two alternatively spliced isoforms of human ketohexokinase, hepatic KHK-C and peripheral KHK-A, and of the ternary complex of KHK-A with the substrate fructose and AMP-PNP have been solved. The differences between KHK-A and KHK-C resulting from the spliced region are subtle and affect thermostability and probably flexibility; the mutations causing fructosuria were modelled.

Determining the DUF55-domain structure of human thymocyte nuclear protein 1 from crystals partially twinned by tetartohedry

The structure of the DUF55 domain of human thymocyte nuclear protein 1, which was determined from partially tetartohedrally twinned crystals, is reported.

De novo sulfur SAD phasing of the lysosomal 66.3 kDa protein from mouse

The structure of the lysosomal 66.3 kDa protein, which crystallizes in the monoclinic space group C2, was solved by means of sulfur SAD phasing using data with an R_anom/R_p.i.m. ratio of 1.1.

The structure of the periplasmic thiol–di­sulfide oxidoreductase SoxS from Paracoccus pantotrophus indicates a triple Trx/Grx/DsbC functionality in chemotrophic sulfur oxidation

The structure of SoxS has been solved in oxidized and reduced forms at 2.1 and 1.9 Å resolution, respectively.

Structural insight into nucleotide recognition by human death-associated protein kinase

The crystal structures of DAPK–ADP–Mg²⁺ and DAPK–AMP-PNP–Mg²⁺ complexes were determined at 1.85 and 2.00 Å resolution, respectively. Comparison of the two nucleotide-bound states with apo DAPK revealed localized changes in the glycine-rich loop region that were indicative of a transition from a more open state to a more closed state on binding of the nucleotide substrate and to an intermediate state with the bound nucleotide product.

Advances in the EDM–DEDM procedure

A revised EDM–DEDM algorithm for phase refinement is described, together with its application to proteins, with special attention being given to molecular-replacement cases.

Structure of the G225P/G226P mutant of mouse 3(17)α-hydroxysteroid de­hydrogenase (AKR1C21) ternary complex: implications for the binding of inhibitor and substrate

Structure of the G225P/G226P mutant of mouse 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) ternary complex is reported at 2.1 Å resolution.

The structure of C2b, a fragment of complement component C2 produced during C3 convertase formation

The crystal structure of C2b has been determined at 1.8 Å resolution, which reveals the arrangement of its three complement control protein (CCP) modules. A model for complement component C2 is presented and its conformational changes during the C3-convertase formation are also discussed.

Structure and function of GlmU from Mycobacterium tuberculosis

The crystal structure of M. tuberculosis GlmU has been determined in an unliganded form and in complex with UDP-GlcNAc or GlcN-1-P. NMR-based enzymatic activity assays suggest that the presence of acetyl-coenzyme A has an inhibitory effect on uridyltransferase activity.

Charge-density study on cyclosporine A

Two single-crystal X-ray diffraction data sets for cyclosporine A were measured to high resolution using synchrotron radiation at temperatures of 5 and 90 K. They allowed an accurate determination of its molecular and electronic structure.

Crystallographic evidence for noncoplanar catalytic aspartic acids in plasmepsin II resides in the Protein Data Bank

The structure of uncomplexed plasmepsin II from P. falciparum is shown to contain noncoplanar catalytic aspartic acid residues.

Crystallographic model quality at a glance

The representation of crystallographic model characteristics in the form of a polygon allows the quick comparison of a model with a set of previously solved structures.

A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry. Corrigendum

A corrigendum to the article by Kato et al. [Acta Cryst. (2008). D64, 525–531].