December 2022 issue
Acta Cryst. (2022). D78, 1412-1427
The use of AlphaFold2 predictions for the detection and correction of sequence-register errors among protein structures determined using cryo-EM deposited in the Protein Data Bank is described.
Acta Cryst. (2022). D78, 1428-1438
Serial femtosecond X-ray crystallography of an anaerobically formed catalytic intermediate of copper amine oxidase
Serial femtosecond X-ray crystallography was performed using a liquid-jet system on bacterial copper amine oxidase microcrystals anaerobically premixed with an amine substrate. The structure determined at 1.94 Å resolution revealed catalytic intermediates in two reduced forms, confirming that anaerobic conditions were well maintained throughout the measurements.
PDB reference: catalytic intermediate of copper amine oxidase, 7ynh
Acta Cryst. (2022). D78, 1439-1450
Host lattice display facilitated the crystallographic analysis of a hydrophobic peptide under aqueous conditions.
PDB references: EngBF_L1_E4_v1–ZHAWOC6027, 8a19; EngBF_L1_F11_v1–ZHAWOC6027, 8a1a
Acta Cryst. (2022). D78, 1451-1468
A new method is suggested for the calculation of maps of limited and eventually inhomogeneous resolution as an analytic function of all atomic parameters, including local resolution. Practical details of map calculation are discussed.
Acta Cryst. (2022). D78, 1469-1473