The ManifoldEM method for cryo-EM: a step-by-step breakdown accompanied by a modern Python implementation

The ManifoldEM method for conformational heterogeneity analysis in single-particle cryo-EM was first presented nearly a decade ago; however, its accessibility to a wide audience has been limited. Here, we describe a step-by-step breakdown accompanied by a modern Python implementation with increased usability, enhanced performance and improved accessibility to future feature additions.

Slice'N'Dice: maximizing the value of predicted models for structural biologists

Recent advancements in computational structural modelling have provided structural biologists with access to high-quality predictions that can be used to aid experimental structure determination. Slice'N'Dice is a new application from CCP4 that is designed to help with the preparation of these predictions for the structure-solution process, particularly where the prediction needs to be split in order to better match the experimental structure.

Human dystrophin tandem calponin homology actin-binding domain crystallized in a closed-state conformation

The structure of the N-terminal actin-binding domain of human dystrophin was determined, revealing a closed conformation with the first and second calponin homology domains directly interacting.

Expansion of the diversity of dispersin scaffolds

Five GH20-family dispersins were characterized for activity on aryl glycosides and poly-β-1,6-linked N-acetyl-D-glucosamine oligomers and three crystal structures were solved, including two complexes with bound inhibitors.