Expression, purification and X-ray crystallographic analysis of thioredoxin from Streptomyces coelicolor

The structures of thioredoxins from several species, including man and E. coli, are known. The bacterium S. coelicolor contains three thioredoxins with unknown physiological functions. TrxA from S. coelicolor has been overexpressed, purified and crystallized and its crystal structure determined at 1.5 Å resolution.

Structure of human factor VIIa/tissue factor in complex with a peptide-mimetic inhibitor: high selectivity against thrombin by introducing two charged groups in P2 and P4

The crystal structure of human factor VIIa/soluble tissue factor in complex with a highly selective peptide-mimetic factor VIIa inhibitor has been solved at 2.6 Å resolution. This structure suggests that Asp60 and Lys192 are key residues for achieving high selectivity against thrombin inhibition.

Crystallization of the SH2-binding site of p130Cas in complex with Lck, a Src-family kinase

The major SH2-binding site of p130Cas has been crystallized in complex with the SH3-SH2 regulatory domains of the Src-family kinase Lck.

Crystallization and preliminary X-ray analysis of α-xylosidase from Escherichia coli

α-Xylosidase from E. coli has been crystallized in space group P2₁2₁2₁.

Expression, purification, crystallization and preliminary X-ray analysis of Aeromonas hydrophilia metallo-β-lactamase

Crystallization and preliminary X-ray analysis of the CphA metallo-β-lactamase from A. hydrophilia are described. The crystals belonged to space group P2₁2₁2, with unit-cell parameters a = 40.75, b = 42.05, c = 128.88 Å, and diffract to 1.8 Å.

Crystallization and preliminary X-ray analysis of Mlc from Escherichia coli

Mlc, a transcriptional regulator from E. coli, has been expressed, purified and crystallized. Diffraction data were collected to 2.9 Å from an Se-derivative crystal.

Crystallization and preliminary structure determination of the plant food allergen Pru av 2

Crystals of Pru av 2, the first allergenic thaumatin-like protein, have been obtained and diffracted to 1.6 Å at a synchrotron. Using an annealing protocol, the resolution limit was improved to 1.3 Å:

Isolation and preliminary crystallographic studies of two new phospholipases A₂ from Vipera nikolskii venom

The isolation, characterization and preliminary crystallographic studies of two new toxic PLA₂s from the venom of V. nikolskii, which has only recently been established as a new species, are reported.

Purification, crystallization and preliminary crystallographic analysis of the biotin–protein ligase from Pyrococcus horikoshii OT3

The biotin–protein ligase from P. horikoshii OT3 was overexpressed, purified, crystallized and cocrystallized with biotin, ADP and biotinyl-5′-AMP. The crystals belong to space group P2₁ and diffract to beyond 1.6 Å resolution.

Crystallization and preliminary crystallographic analysis of bacterial fructosyl amino acid oxidase

Crystallization of a fructosyl amino acid oxidase from Corynebacterium sp. 2-4-1 yields two forms: one monoclinic and one tetragonal.

Crystallization and preliminary X-ray crystallographic studies of glutamate racemase from Lactobacillus fermenti

Glutamate racemase from L. fermenti has been overexpressed and crystallized. Diffraction data have been collected to beyond 2.28 Å resolution using a synchrotron-radiation source.

Expression, purification, crystallization and preliminary X-ray analysis of sepiapterin reductase from Chlorobium tepidum

Sepiapterin reductase from C. tepidum has been crystallized by the hanging-drop vapour-diffusion method. Phasing information has been obtained experimentally by MAD using a SeMet derivative.

Crystallization and preliminary structure analysis of the blue laccase from the ligninolytic fungus Panus tigrinus

Blue laccase from the white-rot basidiomycete P. tigrinus, an enzyme involved in lignin biodegradation, has been crystallized. The crystals obtained give diffraction data at 1.4 Å, the best resolution to date for this class of enzymes, which may assist in further elucidation of the catalytic mechanism of multicopper oxidases.

Expression, purification, crystallization and preliminary crystallographic analysis of the PAB0955 gene product

PAB0955 has been expressed, purified and crystallized, and it has been shown that this thermostable protein is dimeric in reductive conditions.

Mimivirus TyrRS: preliminary structural and functional characterization of the first amino-acyl tRNA synthetase found in a virus

Mimivirus, the largest known double-stranded DNA virus, unexpectedly exhibits components of the protein-translation apparatus. The crystallization and functional assay of the viral tyrosyl tRNA synthetase are reported.

Crystallization and preliminary X-ray crystallographic studies of human cyclophilin J

Human cyclophilin J is a member of the cyclophilin family and recent studies have indicated that it plays a role in the formation of liver cancers as a regulation factor or messenger. The preparation, crystallization and preliminary X-ray crystallographic studies of human cyclophilin J are reported.

Crystallization and preliminary X-ray crystallographic studies of the mesophilic xylanase A from Bacillus subtilis 1A1

Crystallization, data collection and initial structure determination of a mesophilic family 11 xylanase from B. subtilis 1A1 are reported.

Crystallization and preliminary X-ray diffraction studies of the pneumococcal teichoic acid phosphorylcholine esterase Pce

The modular choline-binding protein Pce, the phosphorylcholine esterase from S. pneumoniae, has been crystallized by the hanging-drop vapour-diffusion method. A SAD data set from a derivative with a gadolinium complex has been collected to 2.7 Å resolution.

The purification and crystallization of mDia1 in complex with RhoC

An N-terminal construct of mDia1, a member of the diaphanous-related formin family, was overexpressed, purified and crystallized in complex with RhoC. Data collection from native and SeMet crystals is reported.

Crystallization and preliminary X-ray analysis of the aspartic protease plasmepsin 4 from the malarial parasite Plasmodium malariae

Plasmepsin 4 from the malarial parasite P. malariae has been crystallized in complex with a small molecular inhibitor. Preliminary X-ray analysis of the diffraction data collected at 3.3 Å resolution is reported.

Crystallization and preliminary crystallographic data of a Fel d 1 (1+2) construct corresponding to the major allergen from cat

Fel d 1, a major allergen from cat, has been expressed, purified and crystallized. The crystals belong to space group P1 and diffract to 1 Å resolution.

MdaB from Escherichia coli: cloning, purification, crystallization and preliminary X-ray analysis

The crystallization of the enzyme `modulator of drug activity B' (MdaB) from E. coli, which has activity similar to that of mammalian DT-diaphorase, is presented.

Expression, purification, crystallization and preliminary crystallographic analysis of a deblocking aminopeptidase from Pyrococcus horikoshii

Three paralogues of a deblocking aminopeptidase from the hyperthermophilic archeon P. horikoshii have been overexpressed in E. coli, purified and crystallized and exist in different oligomeric states.

Crystallization and preliminary diffraction studies of the C-terminal domain of the DipZ homologue from Mycobacterium tuberculosis

The C-terminal domain of Rv2874, the DipZ homologue from M. tuberculosis, has been expressed, purified and crystallized.

Crystallization and preliminary X-ray diffraction studies of an alcohol de­hydrogenase from the Antarctic psychrophile Moraxella sp. TAE123

A homo-tetrameric psychrophilic alcohol dehydrogenase from the Antarctic psychrophile Moraxella sp. TAE123 has been crystallized. Its molecular structure is under investigation.

Cloning, expression, purification, crystallization and preliminary crystallographic study of the protein module (BIV2-Helix) in the fusion core of bovine immunodeficiency-like virus (BIV) gp40

A predicted protein module BIV2-Helix of BIVgp40 has been expressed in Escherichia coli and purified by chromatography. Recombinant BIV2-Helix was crystallized using the hanging-drop vapour-diffusion technique at 291 K.

Crystallization and preliminary crystallographic analysis of the cellulose biosynthesis-related protein CMCax from Acetobacter xylinum

The cellulose biosynthesis-related protein CMCax from A. xylinum has been purified and crystallized. The crystals of CMCax belong to the primitive hexagonal space group P6₁ or P6₅, with unit-cell parameters a = b = 89.1, c = 94.2 Å.

Crystallization and preliminary X-ray crystallographic studies of XynX, a family 10 xylanase from Aeromonas punctata ME-1

XynX, a family 10 xylanase from A. punctata ME-1, was crystallized by the hanging-drop vapour-diffusion method. The crystals diffracted to beyond 1.8 Å resolution.