Editorial

Atomic resolution (0.97 Å) structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A₂

The crystal structure of a triple mutant (K53,56,121M) of bovine pancreatic phospholipase A₂ has been solved at atomic resolution (0.97 Å) and the refined model features the presence of a second calcium ion and a chloride ion.

Sequence-induced trimerization of phospholipase A₂: structure of a trimeric isoform of PLA₂ from common krait (Bungarus caeruleus) at 2.5 Å resolution

The structure of a novel trimeric isoform of phospholipase A₂ has been determined at 2.5 Å resolution. The trimer formation occurs in such a way that the active sites of all the three molecules are fully exposed to the solvent, making the trimer a highly potent enzymatic unit.

Structure of Mesorhizobium loti arylamine N-acetyltransferase 1

The crystal structure of a M. loti arylamine N-acetyltransferase 1 has been determined at 2.0 Å resolution.

Mistletoe lectin I in complex with galactose and lactose reveals distinct sugar-binding properties

The structures of mistletoe lectin I in complex with lactose and galactose reveal differences in binding by the two known sites in subdomains α1 and γ2 and suggest the presence of a third low-affinity site in subdomain β1.

Structure of a putative trans-editing enzyme for prolyl-tRNA synthetase from Aeropyrum pernix K1 at 1.7 Å resolution

The three-dimensional structure of the APE2540 protein from A. pernix K1 has been determined by the multiple anomalous dispersion method at 1.7 Å resolution. The structure includes two monomers in the asymmetric unit and shares structural similarity with the YbaK protein or cysteinyl-tRNA^Pro deacylase from H. influenzae.

Crystallization of leucyl-tRNA synthetase complexed with tRNA^Leu from the archaeon Pyrococcus horikoshii

The leucyl-tRNA synthetase (LeuRS) from P. horikoshii has been overexpressed in Escherichia coli and purified, and cocrystallizations with each of the tRNA^Leu isoacceptors have been attempted. Cocrystals were obtained by the hanging-drop vapour-diffusion method, but only when the tRNA^Leu isoacceptor with the anticodon CAA was used.

Overproduction, purification and preliminary X-ray diffraction analysis of a sulfotransferase from Mycobacterium tuberculosis H37Rv

A sulfotransferase from M. tuberculosis was crystallized and preliminarily analyzed using X-ray diffraction.

Crystallization and preliminary X-ray characterization of D-3-hydroxybutyrate de­hydrogenase from Pseudomonas fragi

D-3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) from P. flagi has been crystallized by the hanging-drop method.

Crystallization of the glycogen-binding domain of the AMP-activated protein kinase β subunit and preliminary X-ray analysis

The glycogen-binding domain of the AMP-activated kinase β subunit has been crystallized in the presence of β-cyclodextrin. The structure has been determined by single isomorphous replacement and threefold averaging using in-house X-ray data collected from selenomethionine-substituted protein.

Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii

Crystals of P. horikoshii NikR have been obtained in the presence and absence of Ni²⁺ and characterized by X-ray diffraction.

Purification and crystallization of a trimodular complex comprising the type II cohesin–dockerin interaction from the cellulosome of Clostridium thermocellum

A trimodular complex comprising the type II cohesin–dockerin interaction from the cellulosome of C. thermocellum has been purified and crystallized by the hanging-drop vapour-diffusion method. A native crystal and a selenomethionine derivative have been analyzed using X-ray diffraction.

Preliminary crystallographic analysis of sugar cane phosphoribosylpyrophosphate synthase

X-ray diffraction data have been collected from crystals of recombinant sugar cane phosphoribosylpyrophosphate synthase (PRS) and analysis has revealed its quaternary structure, localizing this PRS into the class of enzymes forming an hexameric oligomer of 223 kDa.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of propionate kinase (TdcD) from Salmonella typhimurium

Propionate kinase (TdcD) from S. typhimurium has been expressed, purified and crystallized. A diffraction data set has been collected to 2.2 Å resolution.

Purification, crystallization and preliminary crystallographic analysis of the vacuole-type ATPase subunit E from Pyrococcus horikoshii OT3

The E subunit of vacuole-type ATPase from P. horikoshii OT3 was overexpressed, purified and crystallized. The native crystals diffracted X-rays to 1.85 Å resolution.

Crystallization and preliminary X-ray diffraction studies of the glutaminyl cyclase from Carica papaya latex

The glutaminyl cyclase isolated from C. papaya latex has been crystallized using the hanging-drop method. Diffraction data have been collected at ESRF beamline BM14 and processed to 1.7 Å resolution.

Crystallization and preliminary X-ray analysis of the GST-fused human Bri3 N-terminal domain

The crystallization of the polyproline-rich polypeptide from human Bri3 overexpressed as a GST-fusion protein in Escherichia coli is presented.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of pantothenate kinase from Mycobacterium tuberculosis

Pantothenate kinase, the first enzyme of the universal coenzyme A biosynthetic pathway, from M. tuberculosis H37Rv has been cloned, expressed, purified and X-ray analysed in two different crystal forms.

Crystallization and preliminary crystallographic analysis of endo-1,3-β-glucanase from Arthrobacter sp.

Endo-1,3-β-glucanase from Arthrobacter sp. has been crystallized and X-ray diffraction data have been collected to 1.66 Å resolution.

Crystallization and preliminary diffraction analysis of a group I ribozyme from bacteriophage Twort

A group I self-splicing RNA has been synthesized and cocrystallized with a four-nucleotide product RNA. Iodination of the product RNA produces a heavy-atom derivative suitable for structure determination.

Crystallization and preliminary X-ray crystallographic analysis of agkicetin-C from Deinagkistrodon acutus venom

The crystallization and preliminary crystallographic analysis of agkicetin-C, a well known platelet glycoprotein Ib (GPIb) antagonist from the venom of Deinagkistrodon acutus found in Anhui Province, China is reported.

Four crystal forms of a Bence-Jones protein

Four crystal forms have been grown and characterized by X-ray diffraction of a Bence-Jones protein collected from the urine of a multiple myeloma patient more than 40 y ago. The trigonal crystal form may shed some light on the formation of fibrils common to certain storage diseases.

Crystallization and preliminary X-ray studies on the reaction center–light-harvesting 1 core complex from Rhodopseudomonas viridis

The reaction center–light-harvesting 1 core complex from R. viridis was crystallized and X-ray diffraction data were collected to 8.0 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds

A lectin from C. maritima was crystallized using the vapour-diffusion method and crystals diffracted to 2.1 Å resolution. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%, refinement is in progress.

Expression, purification, crystallization and preliminary crystallographic study of a potential metal-dependent hydrolase with cyclase activity from Thermoanaerobacter tengcongensis

Crystallization and preliminary crystallographic study of a potential metal-dependent hydrolase with cyclase activity from T. tengcongensis.

Crystallization and preliminary crystallographic studies of the copper-binding domain of the amyloid precursor protein of Alzheimer's disease

The binding of Cu²⁺ ions to the copper-binding domain of the amyloid precursor protein of Alzheimer's disease reduces the production of the amyloid β peptide, which is centrally involved in Alzheimer's disease. Structural studies of the copper-binding domain will provide a basis for structure-based drug design that might prove useful in treating this devastating disease.

Expression, purification, crystallization and preliminary crystallographic analysis of human Pim-1 kinase

Pim kinases, belong to a distinctive serine/threonine protein-kinase family and are involved in cytokine-induced signal transduction and the development of lymphoid malignancies. Human Pim-1 kinase has been cloned, expressed and crystallized

Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45

The trehalulose synthase MutB from P. mesoacidophila MX-45 has been crystallized in two different crystal forms and diffraction data have been collected to 1.6 and 1.8 Å, respectively.

Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu

Bacteriophage Mu baseplate protein gene product 44 was crystallized. The crystal belongs to space group R3, with unit-cell parameters a = b = 126.6, c = 64.2 Å.

Crystallization and preliminary crystallographic study of a recombinant predicted acetamidase/formamidase from the thermophile Thermoanaerobacter tengcongensis

A predicated acetamidase/formanidase from the archaeon T. tengcongensis and its SeMet substitute have been crystallized and undergone preliminarily crystallographic studies including MAD data collection.

Crystallization and preliminary X-ray crystallographic study of disproportionating enzyme from potato

Disproportionating enzyme from potato was crystallized and preliminarily analyzed using X-ray diffraction.

Preliminary crystallographic studies of glucose de­hydrogenase from the promiscuous Entner–Doudoroff pathway in the hyperthermophilic archaeon Sulfolobus solfataricus

A glucose dehydrogenase from the hyperthermophilic archaeon S. solfataricus has been crystallized and subjected to preliminary crystallographic analysis.

Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii

The first crystallographic study of a sucrose phosphate synthase from H. orenii, an organism that is both thermophilic and halophilic, is reported. The protein crystal diffracts X-rays to 3.01 Å.

Crystallization and preliminary X-ray diffraction studies of a catechol-O-methyltransferase/inhibitor complex

Catechol-O-methyltransferase has been co-crystallized with a novel inhibitor, which has potential therapeutic application in the Parkinson's disease therapy.

Crystallization and preliminary X-ray crystallographic analysis of MbtI, a protein essential for siderophore biosynthesis in Mycobacterium tuberculosis

MbtI, the putative isochorismate synthase essential for siderophore biosynthesis in M. tuberculosis, has been crystallized. Diffraction data have been collected to 1.8 Å resolution.

Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants

The production, crystallization and characterization of three inactive mutants of penicillin V acylase from B. sphaericus in their respective precursor and processed forms are reported. The space groups are different for the native enzyme and the mutants.

Crystallization and preliminary X-ray crystallographic analysis of osteoclast-stimulating factor

Crystals of human osteoclast-stimulating factor were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. The crystals are primitive orthorhombic and belong to P222 or a related space group, with unit-cell parameters a = 38.1, b = 54.9, c = 64.7 Å.

Preliminary crystallographic analysis of the antibiotic discharge outer membrane lipoprotein OprM of Pseudomonas aeruginosa with an exceptionally long unit cell and complex lattice structure

The OprM subunit of the MexAB-OprM efflux pump in P. aeruginosa is an outer membrane-anchored lipoprotein. OprM crystals have been grown at 293 K in the presence of 2-methyl-2,4-propanediol and a combination of surfactants and diffracted to 2.56 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of human growth and differentiation factor 5 (GDF-5)

Crystals of human growth and differentiation factor 5 are trigonal, belonging to space group P3₁21 or its enantiomer, with one molecule per asymmetric unit and diffract to 2.2 Å resolution.

On the purification and preliminary crystallographic analysis of iso­quinoline 1-oxidoreductase from Brevundimonas diminuta 7

Crystallization of isoquinoline 1-oxidoreductase from B. diminuta was achieved using two different crystallization buffers. Streak-seeding and cross-linking were essential to obtain well diffracting crystals. Suitable cryo-conditions were found and a structure solution was obtained by molecular replacement.

Crystallization and preliminary X-ray characterization of a lectin from Cicer arietinum (chickpea)

The crystallization and characterization of a lectin isolated and purified from C. arietinum and possessing complex sugar specificity is reported.

Preliminary characterization of two different crystal forms of acylphosphatase from the hyperthermophile archaeon Sulfolobus solfataricus

S. solfataricus acylphosphatase has been expressed, purified and crystallized in two different crystal forms. Preliminary characterization of a triclinic and a monoclinic crystal form is reported and data were collected to 1.27 and 1.90 Å, respectively.

Crystallization and preliminary X-ray analysis of coagulation factor IX-binding protein from habu snake venom at pH 6.5 and 4.6

Crystals of habu coagulation factor IX-binding protein have been obtained at pH 6.5 and 4.6 and characterized by X-ray diffraction.

Crystallization and preliminary X-ray crystallographic analysis of peptide de­formylase (PDF) from Bacillus cereus in ligand-free and actinonin-bound forms

Peptide deformylase (PDF) from B. cereus has been overexpressed, purified and crystallized in ligand-free and actinonin-bound forms. Diffraction data have been collected from these crystals to 1.7 and 2.0 Å resolution, respectively.

Purification, crystallization and preliminary X-ray diffraction analysis of the Kelch-like motif region of mouse Keap1

Keap1-DC (Kelch/double-glycine repeat and C-terminal region) of mouse Keap1 has been overexpressed in E. coli, purified and crystallized using the vapour-diffusion method.

Crystallographic analysis of murine constitutive androstane receptor ligand-binding domain complexed with 5α-androst-16-en-3α-ol

The purification and structure determination of the murine constitutive androstane receptor bound to its inverse agonist/antagonist androstenol is described.

Notes for authors