The copper-containing amine oxidase from Arthrobacter globiformis: refinement at 1.55 and 2.20 Å resolution in two crystal forms

The previously reported structure of the copper-containing amine oxidase from A. globiformis has been refined in two different crystal forms at resolutions of 1.55 and 2.20 Å.

Expression, purification, crystallization and structure of human adipocyte lipid-binding protein (aP2)

The crystal structure of human adipocyte lipid-binding protein (aP2) with a bound palmitate is reported at 1.5 Å resolution.

Fortuitous structure determination of `as-isolated' Escherichia coli bacterioferritin in a novel crystal form

E. coli bacterioferritin was crystallized in a novel crystal form from different conditions and the structure was solved. The crystals belonged to space group P2₁3 and diffracted to a resolution of 2.5 Å.

Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226

Crystal structure of the ligand-binding domain of androgen receptor in complex with LGD2226.

Structures of PmSOD1 and PmSOD2, two superoxide dismutases from the protozoan parasite Perkinsus marinus

PmSOD1 and PmSOD2, two superoxide dismutases from the protozoan parasite P. marinus, have been crystallized and their structures have been solved to 1.8 and 2.3 Å resolution, respectively.

Structure of the orthorhombic form of human inosine triphosphate pyrophosphatase

X-ray crystallographic analysis of human inosine triphosphate pyrophosphohydrolase provided the secondary structure and active-site structure at 1.6 Å resolution in an orthorhombic crystal form. The structure gives a framework for future structure–function studies employing site-directed mutagenesis and for the identification of substrate/product-binding sites.

The nucleotide-binding site of Aquifex aeolicus LpxC

The structure of UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) in complex with UDP is reported. The complex allows for a description of how the enzyme recognizes and binds a nucleotide moiety and enables the construction of an LpxC-substrate model.

Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form

The crystallization of GADPH from S. oleracea in two different space groups has been accomplished using GAPDH samples with unusually low purity levels. One crystal form is the same as a previously reported form, while the second represents a new form. The quality of the diffraction allowed the structure to be determined at a 3 Å resolution limit.

Crystallization, preliminary crystallographic analysis and phasing of the thio­sulfate-binding protein SoxY from Chlorobium limicola f. thio­sulfatophilum

SoxY from C. limicola f. thiosulfatophilum is involved in thiosulfate metabolism. Crystallization, preliminary crystallographic analysis and phasing of this protein are presented.

Purification, crystallization and preliminary X-ray analysis of the glucosamine-6-phosphate N-acetyltransferase from human liver

Glucosamine-6-phosphate N-acetyltransferase from human liver was expressed, purified and crystallized. Diffraction data have been collected to 2.6 Å resolution.

New crystal forms of Diocleinae lectins in the presence of different dimannosides

The crystallization and preliminary X-­ray data of Canavalia gladiata lectin (CGL) and C. maritima lectin (CML) complexed with Man(α1-2)Man(α1)OMe, Man(α1-3)Man(α1)OMe and Man(α1-4)Man(α1)OMe in two crystal forms [the complexes with Man(α1-3)Man(α1)OMe and Man(α1-4)Man(α1)OMe crystallized in space group P3₂ and those with Man(α1-2)Man(α1)OMe crystallized in space group I222], which differed from those of the native proteins (P2₁2₁2 for CML and C222 for CGL), are reported.

Crystallization and preliminary X-ray diffraction analysis of the Bacillus subtilis replication termination protein in complex with the 37-base-pair TerI-binding site

A preparation of replication terminator protein (RTP) of B. subtilis and a 37-­base-pair TerI sequence (comprising two binding sites for RTP) has been purified and crystallized.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of α-11 giardin from Giardia lamblia

α-11 giardin from the intestinal protozoan parasite, G. lamblia has been cloned, expressed, purified and crystallized under two different conditions and in two different space groups. Crystals from the first condition diffracted to 1.1 Å and belong to a primitive orthorhombic space group and crystals obtained in the second condition diffracted to 2.93 Å and belong to a primitive monoclinic space group.

Cloning, crystallization and preliminary X-ray studies of XC2981 from Xanthomonas campestris, a putative CutA1 protein involved in copper-ion homeostasis

A probable copper-ion tolerance protein from the plant pathogen X. campestris has been overexpressed in E. coli, purified and crystallized.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapA (Rv2753c) from Mycobacterium tuberculosis

M. tuberculosis dihydrodipicolinate synthase, the enzyme that catalyzes the first unique reaction in the L-lysine biosynthesis pathway, has been cloned, expressed, purified and crystallized and the crystals have been characterized by X-ray diffraction.

Purification and crystallization of the human EF-hand tumour suppressor protein S100A2

Human recombinant tumour suppressor S100A2 and a mutant lacking cysteine residues have been purified and crystallized. Only the crystals of the mutant protein diffracted to appropriate resolution and a complete data set was recorded at 1.7 Å.

Crystallization of Ranasmurfin, a blue-coloured protein from Polypedates leucomystax

A novel blue protein from frog nests has been crystallized.

Expression, crystallization and preliminary X-ray crystallographic analyses of two N-terminal acetyltransferase-related proteins from Thermoplasma acidophilum

An N-terminal acetyltransferase ARD1 subunit-related protein (Ta0058) and an N-terminal acetyltransferase-related protein (Ta1140) from T. acidophilum were crystallized. X-ray diffraction data were collected to 2.17 and 2.40 Å, respectively.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of phosphopantetheine adenylyltransferase from Enterococcus faecalis

Phosphopantetheine adenylyltransferase from En. faecalis was crystallized and X-ray diffraction data were collected to 2.70 Å resolution.

Preliminary crystallographic characterization of PrnB, the second enzyme in the pyrrolnitrin biosynthetic pathway

Crystals of PrnB, the second enzyme in pyrrolnitrin biosynthesis are reported.

Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)

A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and crystallized.

Crystallization and initial crystallographic characterization of the Corynebacterium glutamicum nitrilotri­acetate monooxygenase component A

The Corynebacterium glutamicum NTA monooxygenase component A protein, which plays the central role in NTA biodegradation, was crystallized. The initial X-ray crystallographic characterization is reported.

Crystallization and preliminary X-ray analysis of human S100A13

Human S100A13 protein was cloned, expressed, purified and crystallized by the hanging-drop vapour-diffusion method. The crystals obtained belonged to space group P2₁2₁2₁ and diffracted to a resolution of 1.8 Å.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative transposase from Thermoplasma acidophilum encoded by the Ta0474 gene

A putative transposase from T. acidophilum encoded by the Ta0474 gene was crystallized and X-ray diffraction data were collected to 1.78 Å.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the regulator AcrR from Escherichia coli

The transcriptional regulator AcrR from Escherichia coli has been cloned, overexpressed, purified and crystallized and X-ray diffraction data have been collected to a resolution of 2.5 Å.

Crystallization and preliminary X-ray analysis of the aromatic prenyltransferase CloQ from the clorobiocin biosynthetic cluster of Streptomyces roseochromogenes

An aromatic prenyltransferase (CloQ) from S. roseochromogenes that is implicated in clorobiocin biosynthesis has been crystallized in space group I4₁22. X-ray data to 2.2 Å resolution were collected in-house.

Crystallization and preliminary X-ray diffraction analysis of phospholipid-bound Sfh1p, a member of the Saccharomyces cerevisiae Sec14p-like phosphatidylinositol transfer protein family

Yeast Sfh1p, a close homolog of the Sec14p phosphatidylinositol transfer protein, was crystallized in the absence of detergent. X-ray data have been collected to 2.5 Å.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of mitochondrial thioredoxin Trx3 from Saccharomyces cerevisiae

Crystals of mitochondrial thioredoxin Trx3 from S. cerevisiae were obtained and diffraction data were collected to 2.0 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of two N-terminal fragments of the DNA-cleavage domain of topoisomerase IV from Staphylococcus aureus

The crystallization and data collection of topoisomerase IV from S. aureus is described. Phasing by molecular replacement proved difficult owing to the presence of translational NCS and strategies used to overcome this are discussed.