Structure of the apo form of the catabolite control protein A (CcpA) from Bacillus megaterium with a DNA-binding domain

Crystal structure analysis of the apo form of catabolite control protein A reveals the three-helix bundle of the DNA-binding domain. In the crystal packing, this domain interacts with the binding site for the corepressor protein.

Structure of the buffalo secretory signalling glycoprotein at 2.8 Å resolution

The crystal structure of a signalling glycoprotein isolated from buffalo dry secretions (SPB-40) has been determined at 2.8 Å resolution. Two unique residues, Tyr120 and Glu269, found in SPB-40 distort the shape of the sugar-binding groove considerably. The water structure in the groove is also different. The conformations of three flexible loops, His188–His197, Phe202–Arg212 and Tyr244–Pro260, also differ from those found in other structurally similar proteins.

Structure determination and analysis of a bacterial chymotrypsin from Cellulomonas bogoriensis

The crystal structure of a secreted chymotrypsin from the alkalophile Cellulomonas bogoriensis has been determined using data to 1.78 Å resolution.

Complex structure of a bacterial class 2 histone de­acetylase homologue with a tri­fluoro­methylketone inhibitor

The crystal structure of HDAH FB188 in complex with a trifluoromethylketone at 2.2 Å resolution is reported and compared to a previously determined inhibitor complex.

Structure of Physarum polycephalum cytochrome b₅ reductase at 1.56 Å resolution

The structure of P. polycephalum cytochrome b₅ reductase, an enzyme which catalyzes the reduction of cytochrome b₅ by NADH, was determined at a resolution of 1.56 Å.

Crystallization and preliminary X-ray crystallographic analysis of Escherichia coli glutaredoxin 2 in complex with gluta­thione and of a cysteine-less variant without gluta­thione

Glutaredoxin 2 from E. coli was cocrystallized with glutathione and data were collected to 1.60 Å. A mutant with the active-site residues Cys9 and Cys12 changed to serine was crystallized in the absence of glutathione and data were collected to 2.4 Å.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the DDX3 RNA helicase domain

Crystals of DDX3 RNA helicase domain have been obtained in a monoclinic form that diffract to 2.2 Å resolution using synchrotron radiation at the ID14-1 ESRF beamline.

Crystallization and preliminary X-ray crystallographic analysis of the heterodimeric crotoxin complex and the isolated subunits crotapotin and phospholipase A₂

Crotoxin, a potent neurotoxin from the venom of the South American rattlesnake Crotalus durissus terrificus, exists as a heterodimer formed between a phospholipase A₂ and a catalytically inactive acidic phospholipase A₂ analogue (crotapotin). Large single crystals of the crotoxin complex and of the isolated subunits have been obtained.

Expression, crystallization and preliminary crystallographic data analysis of filamin A repeats 14–16

The crystallization and crystallographic data analysis of filamin repeats 14–16 are reported.

Crystallization and preliminary crystallographic analysis of a haloalkane dehalogenase, DbjA, from Bradyrhizobium japonicum USDA110

A haloalkane dehalogenase, DbjA, was crystallized by the hanging-drop vapour-diffusion method using PEG 4000 as a precipitant. The crystal belongs to the orthorhombic system, space group P2₁2₁2 and diffracts to 1.75 Å resolution.

Preliminary structural studies on the leucine-zipper homology region of the human protein Bap31

A leucine-zipper with properties as apoptotic regulator in the ER has been crystallized. X-ray data to 2.5 Å resolution were collected, molecular replacement solutions were identified and refinement has been started.

Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid de­carboxylase from Lactobacillus plantarum

The enzyme p-coumaric acid decarboxylase (PDC) from L. plantarum has been recombinantly expressed, purified and crystallized. The structure has been solved at 2.04 Å resolution by the molecular-replacement method.

Cloning, expression, crystallization and preliminary X-ray analysis of the XMT and DXMT N-methyltransferases from Coffea canephora (robusta)

The genes encoding XMT and DXMT, the enzymes from Coffea canephora (robusta) that catalyse the three independent N-methyl transfer reactions in the caffeine-biosynthesis pathway, have been cloned and the proteins have been expressed in Escherichia coli. Both proteins have been crystallized in the presence of the demethylated cofactor S-adenosyl-L-cysteine (SAH) and substrate (xanthosine for XMT and theobromine for DXMT).

Purification, crystallization and preliminary X-ray diffraction study on pyrimidine nucleoside phosphorylase TTHA1771 from Thermus thermophilus HB8

The pyrimidine nucleoside phosphorylase TTHA1771 from T. thermophilus HB8 has been overexpressed, purified and crystallized. The crystals diffract X-rays to 1.8 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe–2S] ferredoxin derived from Pseudomonas sp. strain KKS102

The reduced form of BphA3, a Rieske-type [2Fe–2S] ferredoxin, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions. A molecular-replacement calculation yielded a satisfactory solution.

Crystallization of porcine pancreatic elastase and a preliminary neutron diffraction experiment

To investigate the structural characteristics of a covalent inhibitor bound to porcine pancreatic elastase (PPE), including H atoms and hydration by water, a crystal of porcine pancreatic elastase with its inhibitor was grown to a size of 1.6 mm³ for neutron diffraction study. The crystal diffracted to 2.3 Å resolution with sufficient quality for further structure determination owing to the similar atomic scattering properties of deuterium and carbon.

Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor

A Kunitz-type trypsin inhibitor purified from the seeds of Murraya koenigii has been crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitating agent.

Expression, purification and preliminary X-ray crystallographic studies of the human immunodeficiency virus 1 subtype C protease

Crystals of the human immunodeficiency virus 1 subtype C protease complexed with indinavir and nelfinavir have been grown in the monoclinic space group P2₁ and shown to diffract X-rays to 2.3 Å resolution.

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of molybdopterin synthase from Thermus thermophilus HB8

The molybdopterin synthase from T. thermophilus HB8 was cloned, expressed, purified and crystallized. The crystals belong to space group P2₁ and diffracted to a resolution of 1.64 Å.

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of selenome­thionine-labelled KaiC-like protein PH0186 from Pyrococcus horikoshii OT3

Preliminary crystallographic analysis of KaiC-like protein PH0186 from Pyrococcus horikoshii OT3 is reported.

Expression, purification, crystallization and preliminary crystallographic study of isolated modules of the mouse coactivator-associated arginine methyltransferase 1

Isolated modules of mouse coactivator-associated arginine methyltransferase 1 encompassing the protein arginine N-methyltransferase catalytic domain have been overexpressed, purified and crystallized. X-ray diffraction data have been collected and have enabled determination of the structures by multiple isomorphous replacement using anomalous scattering.

Crystallization and preliminary X-ray crystallographic studies of the biotin carboxyl carrier protein and biotin protein ligase complex from Pyrococcus horikoshii OT3

A truncated form of biotin carboxyl carrier protein containing the C-terminal half fragment (BCCPΔN76) and the biotin protein ligase (BPL) with the mutation R48A (BPL*) or the double mutation R48A K111A (BPL**) were successfully cocrystallized in the presence of ATP and biotin. The BPL*–BCCPΔN76 and BPL**–BCCPΔN76 crystals belong to space group P2₁ and diffract X-rays to 2.7 and 2.0 Å resolution, respectively.

Tyrosyl-tRNA synthetase: the first crystallization of a human mitochondrial amino­acyl-tRNA synthetase

Crystals of human mitochondrial tyrosyl-tRNA synthetase lacking the C-terminal S4-like domain diffract to 2.7 Å resolution and are suitable for structure determination.

Purification, crystallization and preliminary crystallographic analysis of cytochrome P450 203A1 from Rhodopseudomonas palustris

The cytochrome P450 enzyme CYP203A1 from Rhodopseudomonas palustris binds a wide range of highly substituted aromatic compounds and may play an important role in the astonishing metabolic diversity of this organism. Crystals of CYP203A1 that diffract to 2.0 Å resolution have been obtained.

Expression and purification of F-plasmid RepE and preliminary X-ray crystallographic study of its complex with operator DNA

A dimeric inert form of the replication initiator RepE of the F plasmid was expressed, purified and crystallized in complex with the repE operator DNA. Diffraction data were collected to 3.14 Å resolution.

Crystallization, preliminary X-ray diffraction and structure analysis of Thermotoga maritima mannitol de­hydrogenase

T. maritima mannitol dehydrogenase has been crystallized in space group P2₁2₁2₁ with a = 84.43, b = 120.61, c = 145.76 Å. The crystals diffracted to 3.3 Å resolution at the Canadian Light Source.

Crystallization and preliminary X-ray characterization of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv

The phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv was crystallized and diffraction data were collected to 2.8 Å resolution.

Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA

A CcdB homologue from V. fischeri was overexpressed in E. coli and purified. The free protein was crystallized, as were its complexes with fragments of E. coli and V. fischeri gyrase and with the F-plasmid CcdA C-terminal domain.