Structure of the minimized α/β-hydrolase fold protein from Thermus thermophilus HB8

The crystal structure of the minimized α/β-hydrolase fold protein encoded by the gene TTHA1544 from T. thermophilus HB8 has been determined at 2.0 Å resolution.

The structure of allophycocyanin from Thermosynechococcus elongatus at 3.5 Å resolution

The crystal structure of a light-harvesting protein that interacts with photosystem II is reported.

Structure of Bacillus subtilis superoxide dismutase

The crystal structure of MnSOD with four dimers per asymmetric unit was refined to an R factor of 21.1% at 1.8 Å resolution. Differences in the monomer fold and dimer interface relative to the human enzyme can be exploited in the design of selective drugs for Bacillus MnSOD.

Hexammineruthenium(III) ion interactions with Z-DNA

The structure of the complex of the hexanucleotide duplex d(CGCGCA)·d(TGCGCG) with hexammineruthenium(III) ion shows a tautomeric shift in the adenine base and a consequent disruption of the A·T base pair.

Crystallization and preliminary X-ray diffraction studies of the ISC-like [2Fe–2S] ferredoxin (FdxB) from Pseudomonas putida JCM 20004

A vertebrate-type [2Fe–2S] ferredoxin (FdxB), which is probably involved in the iron–sulfur cluster-biosynthesis system of the γ-proteobacterium P. putida JCM 20004, has been crystallized in space group P6₁22. The needle-shaped crystals of recombinant FdxB diffract to 1.90 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray diffraction analysis on the homing endonuclease I-Dmo-I in complex with its target DNA

I-Dmo-I is a well characterized homing endonuclease from the archaeon Desulfurococcus mobilis. The enzyme was cloned and overexpressed in Escherichia coli. Crystallization experiments of I-Dmo-I in complex with its DNA target in the presence of Ca²⁺ and Mg²⁺ yielded crystals that were suitable for X-ray diffraction analysis.

The production and crystallization of the human leukocyte antigen class II molecules HLA-DQ2 and HLA-DQ8 complexed with deamidated gliadin peptides implicated in coeliac disease

The production and crystallization of human leukocyte antigen class II molecules HLA-DQ2 and HLA-DQ8 in complex with deamidated gliadin peptides is reported. Crystals of HLA-DQ2^PQPELPYPQ diffracted to 3.9 Å, while the HLA-DQ8^EGSFQPSQE crystals diffracted to 2.1 Å, allowing structure determination by molecular replacement.

Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal domain of human RSK1 serine/threonine protein kinase

The C-terminal catalytic domain of serine/threonine kinase RSK1 has been crystallized; the crystals diffracted to a resolution of 2.7 Å and belonged to space group P2₁.

An unexpected outcome of surface engineering an integral membrane protein: improved crystallization of cytochrome ba₃ from Thermus thermophilus

Mutation of symmetry-related (in space group P4₃2₁2) glutamate and lysine residues on the water-exposed surface of an integral membrane protein, cytochrome ba₃, from T. thermophilus leads to robust crystallization of the protein in space group P4₁2₁2; crystals of these mutant proteins show X-ray diffraction to molecular resolution.

Crystallization and X-ray diffraction analysis of a novel immune-type receptor from Ictalurus punctatus and phasing by selenium anomalous dispersion methods

A highly diversified novel immune-type receptor from catfish, NITR10, was crystallized to reveal novel mechanisms of immune recognition.

Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin

HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized.

Crystallization and initial crystallographic characterization of a vicilin-type seed storage protein from Pinus koraiensis

In this study, the Korean pine (Pinus koraiensis) vicilin-type 7S seed storage protein was isolated from defatted pine-nut extract and purified by sequential gel-filtration and anion-exchange chromatography. Well diffracting single crystals were obtained by the vapour-diffusion method in hanging drops.

Crystallization and preliminary diffraction studies of CBM3b of cellobio­hydrolase 9A from Clostridium thermocellum

The cloning, expression, purification and crystallization of the CBM3b module of cellobiohydrolase 9A from C. thermocellum is described. The crystals diffract to 2.68 Å.

Crystallization and preliminary X-ray studies of TON_1713 from Thermococcus onnurineus NA1, a putative member of the haloacid dehalogenase superfamily

A putative member of the haloacid dehalogenase superfamily from T. onnurineus has been expressed, purified and crystallized using 1.6 M magnesium sulfate as a precipitant. The crystals belonged to the triclinic space group P1 and diffracted to 1.8 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12

The crystallization of ybfF, a new esterase from E. coli, and the collection of diffraction data to 1.1 Å resolution are reported.

Expression, purification and crystallization of human 5-lipoxygenase-activating protein with leukotriene-biosynthesis inhibitors

The expression, purification and crystallization of human 5-lipoxygenase-activating protein in complex with two leukotriene-biosynthesis inhibitors is decribed. The processes that were used to generate diffraction quality crystals are presented in detail.

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of ScpB (Rv1710) from Mycobacterium tuberculosis

ScpB from M. tuberculosis was crystallized using the sitting-drop vapour-diffusion method in the presence of 2 M NaCl and 10% PEG 6000 at 295 K. X-­ray diffraction data were collected to a maximum resolution of 2.3 Å at a synchrotron beamline.

Crystallization and preliminary crystallographic analysis of the Tob–hCaf1 complex

The antiproliferative complex Tob–hCaf1 was purified and crystallized using the sitting-drop vapour-diffusion method. The crystal diffracted to around 2.6 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12

Crystals scattering to 2.00 Å resolution have been obtained of the ydjA nitroreductase from E. coli K12.

Preliminary X-ray crystallographic studies of a tetrameric phospholipase A₂ formed by two isoforms of crotoxin B from Crotalus durissus terrificus venom

Crotoxin B is a basic phospholipase A₂ found in the venom of C. durissus terrificus and is one of the subunits that constitute crotoxin. Here, the crystallization, X-ray diffraction data collection and molecular-replacement solution of a novel tetrameric complex formed by two dimers of crotoxin B isoforms are presented.

Purification, crystallization and preliminary X-ray diffraction analysis of the plant Rho protein ROP5

Crystals of the plant Rho protein ROP5 from A. thaliana have been obtained that diffract to 1.53 Å resolution.

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7

Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids have been produced which diffract X-rays to ∼3.0 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of the small laccase from Streptomyces coelicolor

The expression, purification and crystallization of the small laccase from S. coelicolor are reported. Diffraction data were collected to 3 Å resolution.

Preliminary neutron and ultrahigh-resolution X-ray diffraction studies of the aspartic proteinase endo­thiapepsin cocrystallized with a gem-diol inhibitor

Three data sets have been collected on endothiapepsin complexed with the gem-diol inhibitor PD-135,040: a high-resolution synchrotron X-ray data set, a room-temperature X-ray data set and a neutron diffraction data set. Until recently, it has been impossible to grow large protein crystals of endothiapepsin with any gem-diol inhibitor that are suitable for neutron diffraction.

Purification, crystallization and preliminary structural analysis of nucleoside diphosphate kinase from Bacillus anthracis

Nucleoside diphosphate kinase from B. anthracis has been crystallized. Preliminary crystallographic analysis shows that there is one monomer in the asymmetric unit of the crystal.

Crystallization, data collection and processing of the chymotrypsin–BTCI–trypsin ternary complex

A ternary complex of the proteinase inhibitor (BTCI) with trypsin and chymotrypsin was crystallized and its crystal structure was solved by molecular replacement.