Cloning, expression and purification of cytochrome c₆ from the brown alga Hizikia fusiformis and complete X-ray diffraction analysis of the structure

The crystal structure of cytochrome c₆ from the brown alga H. fusiformis has been determined at 1.6 Å resolution. The amino-acid sequence and tertiary structure of H. fusiformis cytochrome c₆ were very similar to those of red algal cytochrome c₆ rather than those of green algal cytochrome c₆.

Malonate-bound structure of the glycero­phosphodiesterase from Enterobacter aerogenes (GpdQ) and characterization of the native Fe²⁺ metal-ion preference

A combination of spectroscopic and crystallographic data have been used to identify the native metal-ion preference of GpdQ. These data suggest that GpdQ is a homobinuclear non-haem iron enzyme.

Structure of the Brachydanio rerio Polo-like kinase 1 (Plk1) catalytic domain in complex with an extended inhibitor targeting the adaptive pocket of the enzyme

A crystal structure of zebrafish Plk1 reveals the binding of an extended ATP-competitive inhibitor that targets the adaptive pocket and helps to order the activation segment of the kinase. The conformation of the segment resembles that observed in a nonphosphorylated ligand-free wild-type kinase domain.

Expression, purification and preliminary X-ray diffraction studies of the transcriptional factor PyrR from Bacillus halodurans

The gene-regulation factor PyrR from B. halodurans has been crystallized in two crystal forms. Preliminary crystallographic analysis showed that the protein forms tetramers in both space groups.

Crystallization and preliminary X-ray analysis of L-me­thionine γ-lyase 1 from Entamoeba histolytica

L-Methionine γ-lyase 1, a key enzyme in sulfur-containing amino-acid degradation, from the protozoan parasite E. histolytica was crystallized in a form suitable for X-ray structure analysis.

Crystallization and preliminary X-ray analysis of the inducible lysine de­carboxylase from Escherichia coli

The structure of the decameric inducible lysine decarboxylase from E. coli was determined by SIRAS using a hexatantalum dodecabromide (Ta₆Br₁₂²⁺) derivative. Model building and refinement are under way.

Crystallization of Spätzle, a cystine-knot protein involved in embryonic development and innate immunity in Drosophila melanogaster

Crystallization of the cystine-knot protein Spätzle occurred following serendipitous limited degradation of the pro-Spätzle propeptide during the crystallization experiment.

Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex

Crystals of the soluble ternary GM-CSF receptor complex were obtained which diffracted to a resolution of 3.3 Å.

Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-β-D-xylanase 10B in complex with xylohexaose

The N-terminal moiety of C. thermocellum endo-1,4-β-D-xylanase 10B, comprising a carbohydrate-binding module (CBM22-1) and a GH10 E337A mutant domain, has been crystallized in complex with xylohexaose. The crystals belong to the trigonal space group P3₂21, contain a dimer in the asymmetric unit and diffract to beyond 2.0 Å resolution.

Purification, crystallization and preliminary X-ray analysis of the membrane-bound [NiFe] hydrogenase from Allochromatium vinosum

This article describes the first successful crystallization of a membrane-bound [NiFe] hydrogenase isolated from a photosynthetic organism (A. vinosum). The crystals obtained produced diffraction patterns up to 2.5 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate de­hydrogenase from Helicobacter pylori

Glyceraldehyde-3-phosphate dehydrogenase A has been cloned, expressed and purified. Apoprotein crystals have been grown which diffracted to 1.75 Å resolution and belonged to space group P2₁; holo crystals were grown in the presence of NADP, diffracted to 2.6 Å resolution and belonged to space group P3₂.

Expression, purification, crystallization and preliminary X-ray analysis of an NAD-dependent glyceraldehyde-3-phosphate de­hydrogenase from Helicobacter pylori

Glyceraldehyde-3-phosphate dehydrogenase B from H. pylori has been cloned, expressed, purified and crystallized in the presence of NAD. Crystals of GAPDHB diffracted to 2.8 Å resolution and belonged to space group P6₅22, with unit-cell parameters a = b = 166.1, c = 253.1 Å.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of chlorite dismutase: a detoxifying enzyme producing molecular oxygen

Preliminary X-ray data collection and analysis for crystals of chlorite dismutase, a haem-based enzyme that very effectively reduces chlorite to chloride while producing molecular oxygen, is reported to 2.1 Å resolution.

Purification, identification and preliminary crystallographic studies of a 2S albumin seed protein from Lens culinaris

A 2S albumin from L. culinaris was purified and crystallized and preliminary crystallographic studies were carried out.

Crystallization and preliminary X-ray diffraction studies of maleylacetate reductase from Rhizobium sp. strain MTP-10005

Maleylacetate reductase from Rhizobium sp. strain MTP-10005 has been crystallized using the sitting-drop vapour-diffusion method and microseeding. The crystals contained one dimeric molecule per asymmetric unit and diffracted to 1.79 Å resolution.

Expression, purification and crystallization of Helicobacter pylori L-asparaginase

L-Asparaginase from H. pylori was overexpressed in E. coli, purified and crystallized. The crystals belonged to space group I222, with unit-cell parameters a = 63.6, b = 94.9, c = 100.2 Å and one molecule in the asymmetric unit. A complete data set to 1.6 Å resolution was collected using synchrotron radiation.

Crystallization and preliminary crystallographic analysis of tRNA (m⁷G46) methyltransferase from Escherichia coli

tRNA (m⁷G46) methyltransferase from E. coli was overexpressed, purified and crystallized. Diffraction data were collected to 2.04 Å resolution.

Cloning, expression, purification and preliminary crystallographic analysis of the RNase HI domain of the Mycobacterium tuberculosis protein Rv2228c as a maltose-binding protein fusion

The ribonuclease HI domain of a bifunctional protein Rv2228c-CobC from M. tuberculosis has been crystallized as a fusion protein with maltose-binding protein in a form suitable for high-resolution crystallographic analysis.

Expression, crystallization and preliminary X-ray crystallographic analysis of XometC, a cysta­thionine γ-lyase-like protein from Xanthomonas oryzae pv. oryzae

XometC, a cystathionine γ-lyase-like protein from X. oryzae pv. oryzae and an antibacterial drug-target protein against bacterial blight, was cloned, purified and crystallized. Preliminary X-ray crystallographic analysis of XometC crystals was carried out.

Crystallization and preliminary X-ray analysis of a 1,3–1,4-β-glucanase from Paecilomyces thermophila

The crystal of a 1,3-1,4-β-glucanase produced by Paecilomyces thermophila belongs to the hexagonal space group P6₃22, with unit-cell parameters a = b = 154.54, c = 87.62 Å.

Crystallization and preliminary crystallographic analysis of recombinant immunoglobulin G-binding protein from Streptococcus suis

Crystallization of recombinant IgG-binding protein expressed in Escherichia coli using the hanging-drop vapour-diffusion method is described. The crystals belonged to space group P2₁2₁2₁, with unit-cell parameters a = 38.98, b = 43.94, c = 78.17 Å.

Crystallization and preliminary crystallographic analysis of mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus

The enzyme mannosyl-3-phosphoglycerate synthase from R. xylanophilus has been expressed, purified and crystallized. The crystals belong to the hexagonal space group P6₅22 and diffract to 2.2 Å resolution.

High crystallizability under air-exclusion conditions of the full-length LysR-type transcriptional regulator TsaR from Comamonas testosteroni T-2 and data-set analysis for a MIRAS structure-solution approach

The full-length LysR transcriptional regulator TsaR from C. testosteroni T-2 has been crystallized in two crystal forms and several native and derivative data sets have been collected using synchrotron and in-house X-ray sources.

Preliminary crystallographic data of the three homologues of the thiol–di­sulfide oxidoreductase DsbA in Neisseria meningitidis. Corrigendum

A corrigendum to the paper by Lafaye et al. [Acta Cryst. (2008). F64, 111–114].

Crystallization and preliminary X-ray structural studies of human prouroguanylin. Corrigendum

A corrigendum to the article by Ito et al. [Acta Cryst. (2008). F64, 531–532].