Structure of the thioredoxin-fold domain of human phosducin-like protein 2

The X-ray crystal structure of the Trx-fold domain of hPDCL2 was solved at 2.70 Å resolution and resembled the Trx-fold domain of rat phosducin.

Structure of the ligand-binding domain of the EphB2 receptor at 2 Å resolution

The crystal structure of the ligand-binding domain of a receptor tyrosine kinase EphB2, an important mediator of cell-cell communication, has been determined at a resolution of 2 Å. The structure confirms the induced-fit mechanism for the binding of ligands to EphB receptors.

Structure of human protein kinase CK2α2 with a potent indazole-derivative inhibitor

The crystal structure of human protein kinase CK2α2 with a potent indazole-derivative inhibitor has been determined at 3.2 Å resolution.

Substrate binding induces structural changes in cytochrome P450cam

X-ray structures of ferric cytochrome P450cam partially complexed with the substrate (+)-camphor to two different extents were determined at 1.30–1.35 Å resolution, revealing the protein structures of the substrate-free and substrate-bound forms.

The first structure of a cold-adapted superoxide dismutase (SOD): biochemical and structural characterization of iron SOD from Aliivibrio salmonicida

The crystal structure of iron superoxide dismutase from the cold-adapted organism A. salmonicida has been determined at a resolution of 1.7 Å.

Crystallization and X-ray analysis of human cytoplasmic phenylalanyl-tRNA synthetase

The crystallization and X-ray analysis of recombinant human cytosolic phenylalanyl-tRNA synthetase (hcPheRS) are reported. Diffraction data were collected to 3.3 Å resolution and the hcPheRS structure was determined by the molecular-replacement method using phase information derived from multiwavelength anomalous dispersion data.

Escherichia coli tRNA^Arg acceptor-stem isoacceptors: comparative crystallization and preliminary X-ray diffraction analysis

Various E. coli tRNA^Arg acceptor-stem microhelix isoacceptors have been crystallized and investigated by high-resolution X-ray diffraction analysis.

Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP

The human cytokine IL-22 has been crystallized in complex with its soluble decoy receptor IL-22BP. Diffraction data were collected to 2.75 Å resolution; the crystals belonged to the tetragonal space group P4₁.

Crystallization, data collection and data processing of maltose-binding protein (MalE) from the phytopathogen Xanthomonas axonopodis pv. citri

The Xanthomonas axonopodis pv. citri maltose-binding protein MalE has been crystallized at 293 K using the hanging-drop vapour-diffusion method.

Crystallization and preliminary crystallographic analysis of the second RRM of Pub1 from Saccharomyces cerevisiae

The second RRM of Pub1 from S. cerevisiae was overexpressed, purified and crystallized. Diffraction data were collected to 1.69 Å resolution.

Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea

Degradation-free crystalization of thrombin-digested recombinant His-tagged PsbP protein of photosystem II from Spinacia oleracea resulting in crystals diffracting to 2.06 Å.

Crystallization and preliminary X-ray diffraction analysis of a complex between the electron-transfer partners hexameric Cu-containing nitrite reductase and pseudoazurin

The hexameric Cu-containing nitrite reductase and its electron-donor protein pseudoazurin have been cocrystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 3.3 Å resolution using a synchrotron-radiation source.

Purification, crystallization and preliminary X-ray diffraction studies on avian haemoglobin from pigeon (Columba livia)

Crystallization of pigeon haemoglobin at low pH (5.5) and high ionic concentration (1 M) using the hanging-drop vapour-diffusion method is reported.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the soluble domain of PPA0092, a putative nitrite reductase from Propionibacterium acnes

The soluble domain of a putative copper-containing nitrite reductase from P. acnes has been overexpressed, purified and crystallized. The crystal belonged to space group P2₁3 and diffracted to 2.4 Å resolution.

Preliminary structural investigations of the Eut-L shell protein of the ethanolamine ammonia-lyase metabolosome of Escherichia coli

Preliminary X-ray analysis of crystals of the bacterial microcompartment shell protein Eut-L from Escherichia coli is reported.

Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of a family 101 glycoside hydrolase from Streptococcus pneumoniae

The catalytic module of a family 101 glycoside hydrolase from S. pneumoniae was cloned, recombinantly produced and crystallized.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the small subunit of iso­propylmalate isomerase (Rv2987c) from Mycobacterium tuberculosis

Two C-terminally truncated variants of the small subunit of isopropylmalate isomerase from M. tuberculosis have been cloned, expressed, purified, crystallized and examined by X-ray diffraction.

Heterologous expression, crystallization and preliminary X-ray characterization of CcCel6C, a glycoside hydrolase family 6 enzyme from the basidiomycete Coprinopsis cinerea

Crystallization of CcCel6C was carried out by the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.6 Å resolution from the crystal.

Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2

The C-terminal domain protein of the PB2 subunit of influenza A virus RNA-dependent RNA polymerase was expressed and crystallized and diffraction data were obtained from the crystals.

Crystallization and preliminary X-ray analysis of 2,3-diketo-5-methylthio­pentyl-1-phosphate enolase from Bacillus subtilis

Crystals of the 45.1 kDa functional form of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from B. subtilis diffracted to 2.30 Å resolution.

Crystallization and diffraction of an Lhx4–Isl2 complex

A complex comprising the LIM domains of the LIM-homeodomain protein Lhx4 tethered to a peptide region of Isl2 has been engineered, purified and crystallized. Crystals of this intramolecular complex diffracted to 2.16 Å resolution.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the transcriptional repressor SirR from Mycobacterium tuberculosis H37Rv

The cloning, overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of the transcriptional repressor SirR (staphylococcal iron regulator) from M. tuberculosis are reported.

Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixJ from Anabaena sp. PCC 7120

The chromophore-binding domain of cyanobacteriochrome AnPixJ was overproduced in E. coli, purified and crystallized in its red-absorbing form. Diffraction data were then collected to a resolution of 1.8 Å.

Expression, purification, crystallization and preliminary X-ray studies of the Ebola VP35 interferon inhibitory domain

Native and selenomethionine-labeled crystals of Ebola VP35 interferon inhibitory domain were obtained by the hanging-drop vapor-diffusion method.

Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4

An alkaline alanine racemase from alkaliphilic B. pseudofirmus OF4 was expressed in E. coli and purified. Crystallization and preliminarily X-­ray crystallographic analysis were performed for the recombinant enzyme.

Crystallization and preliminary crystallographic analysis of N-acetyltransferase Mpr1 from Saccharomyces cerevisiae

The yeast N-acetyltransferase that N-acetylates L-azetidine-2-carboxylic acid has been crystallized. Native data were collected to 1.9 Å resolution from a substrate-soaked crystal.

Preliminary X-ray crystallographic analysis of ornithine acetyltransferase (Rv1653) from Mycobacterium tuberculosis

Rv1653, an ornithine acetyltransferase from M. tuberculosis, has been crystallized and diffraction data have been collected to 1.7 Å resolution.

Twinned crystals of adeno-associated virus serotype 3b prove suitable for structural studies

Crystals of adeno-associated virus serotype 3b, a human DNA virus with promise as a vector for gene therapy, have been grown, diffract X-rays to ∼2.6 Å resolution and are suitable for structure determination in spite of twinning.

A preliminary neutron crystallographic study of proteinase K at pD 6.5

Preliminary neutron crystallographic data from the serine protease proteinase K have been recorded using the LADI-III diffractometer at the Institut Laue–Langevin. The results illustrate the feasibility of a full neutron structural analysis aimed at further understanding the catalytic mechanism of proteinase K.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of di­hydrodipicolinate synthase from Bacillus anthracis in the presence of pyruvate

Dihydrodipicolinate synthase (DHDPS) catalyses an important step in lysine biosynthesis. Here, the expression, purification, crystallization and preliminary diffraction analysis to 2.15 Å resolution of DHDPS from B. anthracis soaked with the substrate pyruvate are reported.