Structure of Debaryomyces castellii CBS 2923 phytase

The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 Å resolution.

Structure of the second PDZ domain from human zonula occludens 2

The crystal structure of the second PDZ domain from human zonula occludens 2 has been determined at 1.75 Å resolution.

Structure of D-3-hydroxybutyrate de­hydrogenase prepared in the presence of the substrate D-3-hydroxybutyrate and NAD⁺

The crystal structure of A. faecalis D-3-hydroxybutyrate dehydrogenase prepared in the presence of D-3-hydroxybutyrate and NAD⁺ reveals the substrate/product-binding geometry as the first example which suggests that the catalytic reaction occurs by shuttle movements of a hydrogen negative ion from the substrate to NAD⁺ and from NADH to the product.

Crystallization of the receptor-binding domain of parathyroid hormone-related protein in complex with a neutralizing monoclonal antibody Fab fragment

A Fab fragment of a monoclonal anti-parathyroid hormone-related protein antibody was prepared and its complex with parathyroid hormone-related protein was crystallized. X-ray diffraction data were collected to 2.0 Å resolution.

Crystallization and preliminary X-ray analysis of three dUTPases from Gram-positive bacteria

All organisms examined to date possess a dUTPase that performs the important function of efficiently hydrolyzing dUTP to dUMP in order to prevent the incorporation of dUTP into DNA. Three putative dUTPases from Gram-positive bacteria have been studied in this work.

Isolation, purification, crystallization and preliminary crystallographic studies of chitinase from tamarind (Tamarindus indica) seeds

A 34 kDa chitinase from tamarind (T. indica) seeds was purified, crystallized and characterized using X-ray diffraction.

Crystallographic characterization of a multidomain histidine protein kinase from an essential two-component regulatory system

The multidomain cytoplasmic portion of the histidine protein kinase from an essential two-component signal transduction system has been crystallized and X-ray data have been collected to 2.8 Å resolution.

Crystallization and preliminary crystallographic studies of Mycobacterium tuberculosis DNA gyrase B C-terminal domain, part of the enzyme reaction core

The crystallization of DNA gyrase B C-terminal domain is described.

Crystallization and preliminary X-ray analysis of a novel haloalkane dehalogenase DbeA from Bradyrhizobium elkani USDA94

A novel haloalkane dehalogenase DbeA from B. elkani USDA94 and its mutant variant DbeA1 were crystallized and diffraction data were collected to 2.2 Å resolution.

Crystallization and preliminary crystallographic analysis of fragaceatoxin C, a pore-forming toxin from the sea anemone Actinia fragacea

Fragaceatoxin C, a newly characterized actinoporin from A. fragacea, has been crystallized. Diffraction data were collected to 1.8 Å resolution.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the SH3 domain of human AHI1

The SH3 domain of human AHI1 has been cloned and expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and was crystallized using the sitting-drop vapour-diffusion method at 293 K.

Purification, crystallization and preliminary X-ray analysis of cytochrome P450 219A1 from Novosphingobium aromaticivorans DSM 12444

A 2.4 Å resolution data set was collected from a crystal of CYP219A1. This is the first protein of a new P450 family from the oligotrophic organism N. aromaticivorans which can oxidize steroids and bind sesquiterpenoids.

Overexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519

Recombinant ATP-dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and used to produce crystals that diffracted to 3.09 Å resolution.

Crystallization and preliminary X-ray diffraction studies of vitamin D₃ hydroxylase, a novel cytochrome P450 isolated from Pseudonocardia autotrophica

The purification, crystallization and preliminary X-ray diffraction studies of vitamin D₃ hydroxylase isolated from P. autotrophica are reported.

Crystallization and preliminary crystallographic studies of Helicobacter pylori HugZ, a novel haem oxygenase

The haem oxygenase from H. pylori (HugZ) was overexpressed, purified, reconstituted with haem and crystallized. Selenomethionine-derivatized crystals diffracted X-rays to 1.8 Å resolution.

Improvement of crystal quality by surface mutations of β-lactamase Toho-1

By introducing surface mutations, the crystal quality of a β-lactamase, Toho-1, was drastically improved. The resultant crystals showed no tendency towards merohedral twinning and diffracted to 0.97 Å resolution.

Crystallization and preliminary X-ray analysis of carnein, a serine protease from Ipomoea carnea

The subtilisin-like serine protease carnein was isolated from the latex of the plant I. carnea, purified and crystallized by the hanging-drop vapour-diffusion method. A diffraction data set was collected to 2.0 Å resolution in-house from a single crystal at 110 K.

Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea

A chitinase from the nematophagous fungus C. rosea was overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 1.8 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of human seminal plasma protein PSP94

The human seminal plasma protein PSP94 has been purified from human seminal plasma and crystallized.

Crystallization and preliminary X-ray crystallographic analysis of SMU.412c protein from the caries pathogen Streptococcus mutans

Crystallization of SMU.412c protein from the caries pathogen Streptococcus mutans can easily appear in the condition 2.8 M sodium acetate pH 7.0 and its crystal belongs to space group P4₁2₁2.

Crystallization and preliminary X-ray analysis of a rat aldose reductase-like protein (AKR1B14)

Crystals of AKR1B14 were grown from buffered polyethylene glycol solutions and diffracted to 1.86 Å resolution.

Expression, purification, crystallization and preliminary X-ray diffraction studies of triosephosphate isomerase from methicillin-resistant Staphylococcus aureus (MRSA252)

The cloning, overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of triosephosphate isomerase from MRSA252 is reported.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the TonB-dependent haem outer membrane transporter ShuA from Shigella dysenteriae

ShuA from S. dysenteriae was crystallized in several crystallization conditions containing detergents. Adding heavy atoms during crystallization strongly improved the crystal quality and the resolution limits. Diffraction data were collected at an energy remote from the Pb M absorption edges.

Perdeuteration, purification, crystallization and preliminary neutron diffraction of an ocean pout type III antifreeze protein

Perdeuterated type III antifreeze protein has been expressed, purified and crystallized. Preliminary neutron data collection showed diffraction to 1.85 Å resolution from a 0.13 mm³ crystal.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of YvoA from Bacillus subtilis

YvoA from B. subtilis, a member of the poorly understood HutC family of bacterial transcription factors, has been recombinantly produced, characterized as a homodimer in solution and crystallized in space group C2.

Crystallization and preliminary X-ray analysis of D-2-hydroxyacid de­hydrogenase from Haloferax mediterranei

The D-2-hydroxyacid dehydrogenase from Haloferax mediterranei has been crystallized in two different forms. Diffraction data have been collected to 1.9 Å resolution for the non-productive ternary complex of the enzyme and to 2.7 Å for the selenomethionyl derivative.

Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis

The crystallization of xylose reductase from C. tropicalis is reported.

Expression, purification and preliminary X-ray crystallographic analysis of the chicken MHC class I molecule YF1*7.1

The chicken classical MHC class I antigen YF1*7.1 was crystallized together with β₂-microglobulin but without a peptide ligand. Crystals diffracted synchrotron radiation to 1.32 Å and belonged to the monoclinic space group P2₁.

Purification, crystallization and preliminary X-ray analysis of NtdA, a putative pyridoxal phosphate-dependent aminotransferase from Bacillus subtilis

NtdA was crystallized using the microbatch method. The crystals diffracted to beyond 2.3 Å resolution using synchrotron radiation.