Structure of pig heart citrate synthase at 1.78 Å resolution

The structure of pig heart citrate synthase was solved at 1.78 Å resolution. Cys184 was inadvertently modified through disulfide exchange with a component of the crystallization mixture.

Structure of N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) from Mycobacterium tuberculosis in a cubic space group

The structure of M. tuberculosis N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) was determined by the molecular-replacement method to 3.4 Å resolution in space group I432 and was refined to a final R_work and R_free of 0.285 and 0.321, respectively.

Structure of full-length ubiquitin-conjugating enzyme E2-25K (huntingtin-interacting protein 2)

Crystal structures of the ubiquitin-conjugating enzyme E2-25K M172A mutant protein at pH 6.5 and pH 8.5 were determined to 1.9 and 2.2 Å resolution, respectively. Examination of the structures revealed domain–domain interactions between the UBC and UBA domains which have not previously been reported.

Structure of the human Nac1 POZ domain

The Nac1 POZ domain resembles the POZ-domain dimers of the POZ-zinc finger transcription factors; the structure will have relevance for the design of therapeutics that target Nac1 function in ovarian cancer.

Geobacillus stearothermophilus 6-phosphogluconate de­hydrogenase complexed with 6-phosphogluconate

The structure of 6-phosphogluconate dehydrogenase from a moderate thermophile, G. stearothermophilus, is presented and compared with those of orthologous enzymes.

Structure of TTHA1623, a novel metallo-β-lactamase superfamily protein from Thermus thermophilus HB8

The crystal structures of TTHA1623 from T. thermophilus HB8 in an iron-bound and a zinc-bound form have been determined to 2.8 and 2.2 Å resolution, respectively.

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of β-keto­acyl-ACP synthase III (FabH) from Xanthomonas oryzae pv. oryzae

The ketoacyl-acyl carrier protein synthase III (KASIII) encoded by the fabH gene of X. oryzae pv. oryzae is responsible for elongation by two C atoms in fatty-acid synthesis. The fabH gene was cloned, the KASIII enzyme was expressed and preliminary crystallographic study was performed with the aim of understanding the reaction mechanism.

Expression, refolding, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgE

The expression, purification, refolding and crystallization of the outer membrane protein AlgE from P. aeruginosa is described. The crystals diffracted to 3.0 Å resolution.

Production, crystallization and preliminary X-ray diffraction analysis of the allergen Can f 2 from Canis familiaris

The recombinant form of the allergen Can f 2 from C. familiaris was produced, isolated and crystallized in two different forms. Preliminary X-ray diffraction analyses are reported for the two crystal forms of Can f 2.

Crystallization and preliminary X-ray crystallographic analysis of a novel histidinol-phosphate phosphatase from Thermococcus onnurineus NA1

The TON_0887 gene product was purified and crystallized at 295 K. A 2.2 Å resolution data set was collected using synchrotron radiation.

Crystallization and preliminary X-ray analysis of gluta­thione transferases from cyanobacteria

Glutathione S-transferases (GSTs) are a group of detoxifying enzymes that are found in animals, plants and microorganisms. Here, the crystallizations of two cyanobacterial GSTs are reported with the aim of determining their atomic structures.

Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana

The crystallization of the receiver domain of the histidine kinase CYTOKININ-INDEPENDENT1 from A. thaliana is described. The crystals diffracted to 2.0 Å resolution.

Crystallization of human complement component C3b in the presence of a staphylococcal complement-inhibitor protein (SCIN)

The crystallization and results of multiwavelength anomalous diffraction studies of a recombinant C3-inhibitory fragment of Efb from S. aureus are reported.

Crystallization and preliminary X-ray analysis of a bifunctional catalase-phenol oxidase from Scytalidium thermophilum

The bifunctional enzyme catalase-phenol oxidase from S. thermophilum was crystallized by the hanging-drop vapour-diffusion method in space group P2₁ and diffraction data were collected to 2.8 Å resolution.

Preliminary X-ray crystallographic studies of yeast Get3

Get3 is a cytosolic ATPase which can recognize and bind the transmembrane domain of tail-anchored proteins. Yeast Get3 has been crystallized and the crystals diffracted to 2.7 Å resolution using a synchrotron X-ray source.

Expression, purification, crystallization and preliminary crystallographic analysis of laminin-binding protein (Lmb) from Streptococcus agalactiae

Laminin-binding protein from S. agalactiae was expressed, purified and crystallized and X-ray diffraction data were collected to 2.5 Å resolution.

Preliminary joint neutron and X-ray crystallographic study of human carbonic anhydrase II

A joint X-ray and neutron crystallographic study has been initiated to determine the specific water network and the protonation states of the hydrophilic residues that coordinate it in human carbonic anhydrase II.

Crystallization and crystallographic analysis of Bacillus subtilis xylanase C

XynC, a glycosyl hydrolase family 5 xylanase from B. subtilis, has been purified and crystallized with the primary goal of structural determination and characterization of a second xylanase from this large catalytically diverse glycosyl hydrolase family.

Purification, crystallization and data collection of the apoptotic nuclease endonuclease G

Mouse endonuclease G without its mitochondrial localization signal (amino-acid residues 1–43) was successfully overexpressed, purified and crystallized using a microbatch method under oil.

Crystallization and preliminary X-ray diffraction analysis of the tRNA-modification enzyme GidA from Aquifex aeolicus

A. aeolicus GidA has been crystallized in two different crystal forms: forms I and II. X-ray diffraction data were collected to 3.2 and 2.3 Å resolution, respectively, using a synchrotron-radiation source.

Crystallization and preliminary X-ray diffraction analysis of a protease-resistant mutant form of human galectin-8

A protease-resistant mutant form of human galectin-8 has been crystallized and diffraction data have been collected to 3.4 Å resolution.

Crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein Algp7 isolated from Sphingomonas sp. A1

The crystallization of an alginate-binding protein isolated from Sphingomonas sp. A1 and the results of preliminary crystallographic analysis of this protein are presented.

Purification and preliminary X-ray crystallographic studies of β-microseminoprotein from human seminal plasma

The preliminary X-ray data of β-microseminoprotein isolated from human seminal plasma at 2.4 Å resolution are reported.

Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase

The first crystallographic study of a plant subtilase, SBT3 from S. lycopersicum, is reported.

Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9

The gene that encodes human coronavirus HKU1 nonstructural protein 9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials.

Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1

Human ADP-ribosylhydrolase 1, which cleaves the glycosidic bond between ADP-ribose and specific Arg residues in proteins, has been cloned, expressed, purified and crystallized.

Crystallization of a nonclassical Kazal-type Carcinoscorpius rotundicauda serine protease inhibitor, CrSPI-1, complexed with subtilisin

A recombinant serine protease inhibitor, CrSPI-1, from horseshoe crab, which inhibits subtilisin with a K_i of 10⁻⁹ M, has been cloned, expressed, purified and cocrystallized with subtilisin. The crystals diffracted to 2.6 Å resolution.

Purification, crystallization and preliminary X-ray analysis of isocitrate de­hydrogenase kinase/phosphatase from Escherichia coli

Isocitrate dehydrogenase kinase/phosphatase has been crystallized in three different crystal forms. Data were collected from each crystal form for structure determination.