Structure of AmpC β-lactamase (AmpC^D) from an Escherichia coli clinical isolate with a tripeptide deletion (Gly286-Ser287-Asp288) in the H10 helix

The structure of AmpC β-lactamase (AmpC^D) from E. coli with a tripeptide deletion (Gly286-Ser287-Asp288) is reported.

ATP-dependent DNA ligase from Archaeoglobus fulgidus displays a tightly closed conformation

The crystal structure of an ATP-dependent DNA ligase from A. fulgidus has been determined. It resembles, but shows some variations from, the closed conformation of DNA ligase from P. furiosus.

Comparison of GFL–GFRα complexes: further evidence relating GFL bend angle to RET signalling

The second crystal structure of the GDNF-GFRα1 complex provides further evidence that GFL signalling through RET is determined by the bend angle in the GFL.

Expression, crystallization and preliminary crystallographic analysis of the PAS domain of RsbP, a stress-response phosphatase from Bacillus subtilis

The PAS domain of RsbP, a stress-response protein from B. subtilis, was crystallized using the sitting-drop vapour-diffusion method. The crystals belonged to space group P2₁ and diffraction data were collected to a resolution of 1.6 Å.

Purification, crystallization and preliminary X-ray analysis of L-sorbose reductase from Gluconobacter frateurii complexed with L-sorbose or NADPH

NADPH-dependent L-sorbose reductase from G. frateurii (SR) was expressed, purified and crystallized with L-sorbose or NADPH using the sitting-drop vapour-diffusion method. Crystals of the SR–L-sorbose complex and SR–NADPH complex diffracted X-rays to 2.38 and 1.90 Å resolution, respectively.

Crystallization and preliminary crystallographic studies of putative RNA 3′-terminal phosphate cyclase from the crenarchaeon Sulfolobus tokodaii

RNA 3′-terminal phosphate cyclase from the crenarchaeon S. tokodaii has been overexpressed and purified. The apo form, its selenomethionine derivative and their complexes with ATP under different conditions have been crystallized. The structure of the selenomethionine-labelled form has been successfully solved by the Se-MAD method.

Crystallization of Saccharomyces cerevisiae α-mannosidase, a cargo protein of the Cvt pathway

Vacuolar α-mannosidase, a cargo protein of the cytoplasm-to-vacuole targeting pathway, has been expressed, purified and crystallized.

Cloning, purification, crystallization and preliminary crystallographic analysis of a ribokinase from Staphylococcus aureus

The ribokinase Sa239 from S. aureus has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.9 Å resolution.

Isolation, crystallization and preliminary X-ray analysis of the transamidosome, a ribonucleoprotein involved in asparagine formation

The isolation, crystallization and preliminary X-ray crystallographic data of the transamidosome from T. thermophilus, a ribonucleoprotein particle that provides the translational machinery with Asn-tRNA^Asn, are reported.

Purification, crystallization and preliminary X-ray analysis of the β-lactamase Oih-1 from Oceanobacillus iheyensis

Oih-1, a β-lactamase enzyme isolated from the deep-sea bacterium O. iheyensis, has been crystallized and a complete X-ray diffraction data set has been collected to 1.65 Å resolution.

Crystallization and preliminary X-ray analysis of Escherichia coli RNase G

Full-length E. coli RNase G was overexpressed, purified and crystallized. Diffraction data were collected to a resolution of 3.4 Å.

Preliminary X-ray data analysis of crystalline hibiscus chlorotic ringspot virus

Hibiscus chlorotic ringspot virus is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family. Authentic virus harvested from infected host kenaf leaves was purified and virus crystals were grown in multiple conditions. One of the crystals diffracted to 3.2 Å resolution and allowed the collection of a partial data set.

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of interleukin-4-inducing principle from Schistosoma mansoni eggs (IPSE/alpha-1)

Interleukin-4-inducing principle from S. mansoni eggs (IPSE/alpha-1) was cloned, purified and crystallized using the hanging-drop vapour-diffusion method at 293 K. X-ray diffraction data were collected to 1.66 Å resolution.

Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL

Mouse RANKL and its receptor RANK have been cloned, expressed and purified. Crystals of RANK alone and in complex with RANKL have been obtained from which diffraction data have been collected to 2.0 and 2.8 Å resolution, respectively.

Crystallization and preliminary X-ray diffraction analysis of Salmonella typhimurium uridine phosphorylase complexed with 5-fluorouracil

Uridine phosphorylase from S. typhimurium was expressed and purified and cocrystallized with the drug 5-fluorouracil. The crystals diffracted X-rays to 2.2 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray crystallographic analysis of two dimeric hyperthermostable thioredoxins isolated from Sulfolobus solfataricus

Two thioredoxins (SsTrxA2 and SsTrxA1) isolated from S. solfataricus have been crystallized. Diffraction data have been collected from SsTrxA2 and SsTrxA1 to 1.83 and 1.90 Å resolution, respectively. The structures of both enzymes have been solved by molecular replacement.

Crystallographic characterization of the passenger domain of the Bordetella autotransporter BrkA

A secreted bacterial protein from a human pathogen that mediates serum resistance and adherence was overexpressed, purified, refolded and crystallized. Preliminary X-ray diffraction data are presented.

Crystallization and X-ray diffraction data collection of topoisomerase IV ParE subunit from Xanthomonas oryzae pv. oryzae

The ParE subunit of topoisomerase IV from X. oryzae pv. oryzae was expressed, purified and crystallized. Diffraction data were collected to a resolution of 2.15 Å.

Crystallization and preliminary X-ray analysis of the V domain of human nectin-2

Crystals of the V domain of human nectin-2 diffracted to 1.85 Å resolution and were monoclinic, belonging to space group P2₁, with unit-cell parameters a = 52.3, b = 43.9, c = 56.1 Å, β = 118.2°.

Crystallization of Mycobacterium smegmatis me­thionyl-tRNA synthetase in the presence of me­thionine and adenosine

The expression, purification and crystallization of methionyl-tRNA synthetase from Mycobacterium smegmatis. The crystals diffracted to 2.1 Å.

Crystallization and preliminary X-ray studies of the N-domain of the Wilson disease associated protein

The modified N-domain of the Wilson disease protein has been crystallized both in its native form and as a selenomethionine derivative. X-ray diffraction data were collected from the native crystal to 1.7 Å resolution, while the selenomethionine derivative diffracted to 2.7 Å resolution.

Crystallization of the flexible nuclear import receptor importin-β in the unliganded state

Conditions are reported for the crystallization of the flexible nuclear import receptor importin-β. Preliminary X-ray diffraction data indicate that the structure can be solved using molecular replacement.

Overexpression, purification, crystallization and preliminary X-ray studies of Vibrio cholerae EpsG

Recombinant V. cholerae EpsG has been expressed, purified and crystallized. The crystals diffracted to 2.26 Å resolution.

Crystallization and preliminary crystallographic analysis of β-L-arabino­pyranosidase from Streptomyces avermitilis NBRC14893

β-L-Arabinopyranosidase from S. avermitilis NBRC14893 was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to 1.6 Å resolution and belonged to space group P2₁2₁2₁.

Isolation, purification, crystallization and preliminary crystallographic studies of amaryllin, a plant pathogenesis-related protein from Amaryllis belladonna

A novel 15 kDa antifungal protein amaryllin has been crystallized using 30% PEG 8000 as the precipitating agent. The crystals belong to orthorhombic space group I or I2₁2₁2₁ with cell dimensions, a = 48.6, b = 61.9 and c = 79.6–Å.

Crystallization of carbohydrate oxidase from Microdochium nivale

Industrially used carbohydrate oxidase was successfully crystallized in several forms, diffraction data suitable for structural analysis were collected.

Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain

The gene coding for Q4DV70 has been cloned and the protein overexpressed in Escherichia coli with an N-­terminal His tag. Purification of Q4DV70 was carried out by affinity and size-exclusion chromatography and the His tag was removed by TEV protease digestion. Crystals of Q4DV70 were grown using the sitting-drop vapour-diffusion method.

Crystallization and preliminary X-ray analysis of the NADPH-dependent 3-quinuclidinone reductase from Rhodotorula rubra

The NADPH-dependent 3-quinuclidinone reductase from Rhodotorula rubra was expressed, purified, and crystallized and X-ray diffraction data of this crystal were collected to 2.2 Å resolution.

Crystallization and preliminary crystallographic studies of human RIG-I in complex with double-stranded RNA

RIG-I is important in recognizing viral RNA and stimulating the immune system. Human RIG-I protein was crystallized in complex with 18-mer double-stranded RNA to study the recognition mechanism.

Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5

Grx5 from Saccharomyces cerevisiae is a monothiol glutaredoxin that is involved in iron–sulfur cluster biogenesis. Here, yeast Grx5 was cloned and overproduced in Escherichia coli and the purified protein was crystallized using the hanging-drop vapour-diffusion method.