Citations in supplementary material

The problem of undercounting of citations that are published only in supplementary material is studied for the journals Nature, Science, Cell and the Proceedings of the National Academy of Sciences (USA).

Structure of a CRISPR-associated protein Cas2 from Desulfovibrio vulgaris

A 1.35 Å resolution crystal structure of Cas2 from the bacterium Desulfovibrio vulgaris (DvuCas2) is reported.

Structure of Hsp33/YOR391Cp from the yeast Saccharomyces cerevisiae

The crystal structure of Hsp33 from S. cerevisiae was solved at 2.40 Å resolution. Structural comparison showed that Hsp33 has a very similar structure to Hsp31, except for some deviations in helices α2–α3 of the core domain.

Structures of bacterial biosynthetic arginine de­carboxylases

Crystal structures of biosynthetic arginine decarboxylase (ADC, SpeA) from E. coli and C. jejuni are reported.

Structure of the iSH2 domain of human phosphatidylinositol 3-kinase p85β subunit reveals conformational plasticity in the interhelical turn region

The crystal structure of human PI3K p85β iSH2 domain has been determined to 3.3 Å resolution. Comparison with the published structure of the bovine p85β iSH2 domain bound to the influenza A virus nonstructural protein 1 indicates that little or no structural change occurs upon complex formation. Structural analysis of human and bovine p85β iSH2 domains reveals conformational plasticity in the interhelical turn region of the coiled-coil motif.

A new crystal form of human vascular adhesion protein 1

The structure of human vascular adhesion protein 1 in space group C2 with seven molecules in the asymmetric unit has been refined at a resolution of 2.9 Å.

Crystallization and preliminary X-ray analysis of a novel esterase Rv0045c from Mycobacterium tuberculosis

The novel esterase Rv0045c from M. tuberculosis was expressed and purified to homogeneity. The crystals of native and SeMet-labelled Rv0045c protein that were obtained diffracted to resolutions of 2.7 and 3.0 Å, respectively.

Purification, crystallization and preliminary crystallographic analysis of a multiple cofactor-dependent DNA ligase from Sulfophobococcus zilligii

A recombinant multiple cofactor-dependent DNA ligase from S. zilligii has been purified and crystallized. X-ray diffraction data were collected to 2.9 Å resolution and the crystals belonged to space group P1.

Crystallization and preliminary X-ray data collection of the Escherichia coli lipoproteins BamC, BamD and BamE

The cloning, purification and crystallization of the E. coli lipoproteins BamC, BamD and BamE is reported. X-ray diffraction data at high resolution were obtained for each of the proteins or protein domains.

Purification, crystallization and preliminary X-ray crystallographic analysis of the human heat-shock protein 40 Hdj1 and its C-terminal peptide-binding domain

The purification, crystallization and preliminary crystallographic analysis of the C-terminal peptide-binding domain of the human Hsp40 Hdj1 and of full-length Hdj1 are reported.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of intracellular growth locus E (IglE) protein from Francisella tularensis subsp. novicida

The F. tularensis protein IglE from the FPI, which is a component of the type VI-like secretion system, has been crystallized and preliminary X-ray data have been collected.

Crystallization and preliminary X-ray diffraction analysis of a class V chitinase from Nicotiana tabacum

N. tabacum class V chitinase has been crystallized. X-ray diffraction data were collected to 1.2 Å resolution using a synchrotron-radiation source.

Crystallization and preliminary crystallographic analysis of a calcineurin B-like protein 1 (CBL1) mutant from Ammopiptanthus mongolicus

Recombinant calcineurin B-like protein 1 from Ammopiptanthus mongolicus (AmCBL1) was overexpressed, purified and crystallized.

Expression, purification, crystallization and initial X-ray diffraction analysis of thiol peroxidase from Yersinia pseudotuberculosis

Recombinant thiol peroxidase from Y. pseudotuberculosis has been purified and crystallized in three crystal forms.

High-level expression, purification, crystallization and preliminary X-ray crystallographic studies of the receptor-binding domain of botulinum neurotoxin serotype D

The receptor-binding domain of botulinum neurotoxin serotype D was expressed in E. coli using a codon-optimized cDNA. The highly purified protein crystallized in space group P2₁2₁2₁, with unit-cell parameters a = 60.8, b = 89.7, c = 93.9 Å, and the crystals diffracted to 1.65 Å resolution.

Purification, crystallization and preliminary X-ray diffraction analysis of DNA damage response A protein from Deinococcus radiodurans

In this study, a recombinant C-terminally truncated form of D. radiodurans DdrA (DdrA157) comprising the first 157 residues of DdrA has been expressed in Escherichia coli, purified and crystallized.

Improving the diffraction of full-length human selenome­thionyl metavinculin crystals by streak-seeding

Streak-seeding markedly improved the stability, crystal-growth rate and diffraction quality of dilated-cardiomyopathy-associated mutant metavinculin crystals.

Crystallization and preliminary diffraction analysis of Wzi, a member of the capsule export and assembly pathway in Escherichia coli

Wzi is a membrane protein from E. coli thought to be involved in the attachment of capsular polysaccharides to the bacterial surface. This reports describes recombinant Wzi's purification, crystallization and the results of initial diffraction studies.

Preliminary crystallographic analysis of two oligomerization-deficient mutants of the aerolysin toxin, H132D and H132N, in their proteolyzed forms

Information about the intermediate states in the oligomerization process of a pore-forming toxin is lacking. Two oligomerization-deficient mutants of aerolysin, a major virulence factor secreted by A. hydrophila, which is involved in gastrointestinal infections in humans, have been crystallized in their proteolyzed forms and their preliminary X-ray analysis is reported.

Crystallographic studies of the coupling segment NBD94_674–781 of the nucleotide-binding domain of the Plasmodium yoelii reticulocyte-binding protein Py235

The coupling region NBD94_674–781 of Py235 from P. yoelii was cloned, overexpressed, purified and crystallized. The crystals belonged to space group C2 and diffracted to 2.9 Å resolution using synchrotron radiation.

Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp. CY24

AgaB from Pseudoalterromonas sp. CY24 has been crystallized. The crystals of wild-type enzyme and the selenomethionine derivative diffracted to 1.97 and 2.64 Å, respectively.

Preparation and crystallization of the Grb7 SH2 domain in complex with the G7-18NATE nonphosphorylated cyclic inhibitor peptide

The preparation and successful crystallization of the Grb7 SH2 domain in complex with the specific cyclic peptide inhibitor G7-18NATE are reported. This structure is anticipated to reveal the basis of the binding affinity and specificity and to assist with the development of second-generation inhibitors of Grb7, which is involved in cancer progression.

Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein

The crystallization of a specific nanobody against mouse PrP^C and preliminary diffraction analysis of a crystal that diffracted to 1.23 Å resolution are presented.

Crystallization and preliminary X-ray crystallographic analysis of zebrafish prototype galectin Drgal1-L2

Zebrafish Drgal1-L2 was expressed in E. coli, purified and crystallized in the presence of lactose. X-ray diffraction data have been collected to a resolution of 1.5 Å.

Cloning, purification and preliminary crystallographic analysis of cobalamin methyltransferases from Rhodobacter capsulatus

Diffraction from crystals of the cobalamin methyltransferases CobJ, CobM, CobF and CobL (full-length and C-terminal domain) from R. capsulatus is reported; three of these enzymes also catalyse the auxiliary reactions of ring contraction (CobJ), decarboxylation (CobL) and deacylation (CobF).

Crystallization and preliminary X-ray crystallographic analysis of a Mycobacterium tuberculosis ferritin homolog, BfrB

The ferritin homolog, BfrB (Rv3841), from Mycobacterium tuberculosis has been purified, crystallized, and diffraction data were collected to 2.5 Å resolution. Here, preliminary crystallographic characterization and SAXS analyses are reported.

Cloning, expression, purification, crystallization and preliminary X-ray studies of a secreted lectin (Rv1419) from Mycobacterium tuberculosis

A secreted lectin from M. tuberculosis has been crystallized.

Purification, crystallization and halide phasing of a Streptococcus agalactiae backbone pilin GBS80 fragment

The C-terminal fragment of Streptococcus agalactiae (group B streptococcus) major (backbone) pilin GBS80 was purified and crystallized in two different space groups. Single-wavelength anomalous dispersion (SAD) data collected to 2.0 Å resolution on a iodide (NaI) derivative crystal using the home source were used to obtain initial phases.

Purification, crystallization and preliminary X-ray diffraction experiment of nattokinase from Bacillus subtilis natto

Nattokinase, a protein found in high levels in the traditional Japanese food natto, has been reported to have high thrombolytic activity. In the present study, the crystallization of native nattokinase and the collection of X-ray diffraction date from a nattokinase crystal to a resolution of 1.74 Å are reported.

Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of the N-terminal domain of DEAD-box RNA helicase from Staphylococcus aureus strain Mu50

The N-terminal domain of the DEAD-box helicase from S. aureus strain Mu50 was cloned, overexpressed, purified and crystallized. X-ray diffraction data were collected to 2.60 Å resolution.

Crystallization of the Na⁺-translocating NADH:quinone oxidoreductase from Vibrio cholerae

The crystallization of the six-subunit Na⁺-NQR complex from V. cholerae is reported.

Crystallization and preliminary X-ray analysis of 5-keto-D-gluconate reductase from Gluconobacter suboxydans IFO12528 complexed with 5-keto-D-gluconate and NADPH

NADPH-dependent 5-keto-D-gluconate reductase from G. suboxydans IFO12528 (5KGR) was expressed, purified and crystallized with 5-keto-D-gluconate and NADPH using the sitting-drop vapour-diffusion method. Crystals of the 5KGR–NADPH complex and of the 5KGR–NADPH–5-keto-D-gluconate complex diffracted X-rays to 1.75 and 2.26 Å resolution, respectively.

Crystallization and preliminary crystallographic analysis of the ADP-ribosyltransferase HopU1. Corrigendum

A correction to the article by Lin et al. [(2010). Acta Cryst. F66, 932–934].

Crystallization and preliminary crystallographic analysis of the central domain of Drosophila Dribble, a protein that is essential for ribosome biogenesis. Addendum

An addition to the paper by Cheng et al. [(2010), Acta Cryst. F66, 546–548].