High-resolution structure of AKR1a4 in the apo form and its interaction with ligands

Despite its high affinity for NADPH, AKR1a4 crystallized in the apo form, which is very rare for aldo-keto reductase enzymes.

Influence of intermolecular contacts on the structure of recombinant prolidase from Thermococcus sibiricus

The structure of recombinant prolidase from the hyperthermophilic archaeon Thermococcus sibiricus (Tsprol) was determined at 2.6 Å resolution.

Structure of glycerol-3-phosphate de­hydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 Å resolution

The first structure of a glycerol-3-phosphate dehydrogenase from S. cerevisiae, GPD1, is reported at 2.45 Å resolution.

Solution structure of the cold-shock-like protein from Rickettsia rickettsii

The solution structure of the cold-shock-like protein from R. rickettsii, the causative agent of Rocky Mountain spotted fever, is reported.

Structure of the recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the marine invertebrate Stichodactyla helianthus

The recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the Caribbean sea anemone S. helianthus has been crystallized and its structure has been refined to 2.5 Å resolution and compared with known X-ray structures of BPTI-like Kunitz-type inhibitors.

Structure of Escherichia coli RutC, a member of the YjgF family and putative aminoacrylate peracid reductase of the rut operon

The structure of the putative aminoacrylate peracid reductase RutC of the rut operon, a member of the YjgF family, is reported.

Room-temperature X-ray diffraction studies of cisplatin and carboplatin binding to His15 of HEWL after prolonged chemical exposure

Binding of cisplatin to His15 in hen egg-white lysozyme in aqueous media is observed after prolonged chemical exposure for 15 months, in contrast to the lack of binding that was observed after 4 d in a previous study. Binding of carboplatin is seen in greater detail in the case of room-temperature data collection compared with cryo data collection.

In situ X-ray data collection from highly sensitive crystals of Pseudomonas putida PtxS in complex with DNA

The crystallization of both native P. putida transcriptional regulator PtxS and its complex with its DNA recognition sequence using the counter-diffusion method are reported.

Crystallization and preliminary X-ray analysis of the FliH–FliI complex responsible for bacterial flagellar type III protein export

The FliH–FliI complex from the bacterial flagellar type III export apparatus has been expressed, purified and crystallized, and the crystals have been characterized by X-ray diffraction.

Crystallization and preliminary crystallographic studies of the W2 domain of Drosophila melanogaster eukaryotic translation initiation factor 5C domain-containing protein

The crystallization and preliminary crystallographic studies of the carboxy-terminal domain of D. melanogaster eukaryotic translation initiation factor 5C domain-containing protein are reported.

Purification, crystallization and preliminary crystallographic analysis of human cysta­thionine β-synthase

This article describes the crystallization and preliminary crystallographic analysis of a protein construct (hCBS_516–525) that contains the full-length cystathionine β-synthase from Homo sapiens (hCBS) and just lacks amino-acid residues 516–525.

Purification, crystallization and preliminary crystallographic analysis of the full-length cysta­thionine β-synthase from Apis mellifera

This manuscript describes the crystallization of the full-length enzyme cystathionine β-synthase (CBS) from Apis mellifera, which maintains 46% sequence identity with the human homolog. Mutations in CBS cause hereditary homocystinuria in humans.

Purification, crystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A₂ with vasoconstrictor activity from Agkistrodon halys pallas venom

A vasoconstrictor PLA₂ was purified from Agkistrodon halys pallas venom and the preliminary X-ray diffraction analysis had been described.

Purification, crystallization and preliminary X-ray crystallographic analysis of the ETS domain of human Ergp55 in complex with the cfos promoter DNA sequence

The ETS domain of human Ergp55 was purified and crystallized in native, complexes with E74, and cfos promoter DNA sequences. The X-ray intensity data set was collected on ETS–cfos promoter DNA complex crystal at 3.1 Å resolution to analyze the structure by molecular replacement technique.

Crystallization and preliminary crystallographic analysis of 2-aminophenol 1,6-dioxygenase complexed with substrate and with an inhibitor

The crystallization of 2-aminophenol 1,6-dioxygenase in complexes with its substrate and with an inhibitor is reported.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the di­hydroorotase domain of human CAD

Recombinant dihydroorotase domain of human CAD was expressed in E. coli, purified and crystallized. An X-ray diffraction data set was collected to 1.75 Å resolution.

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster

D. melanogaster Zucchini was expressed, purified and crystallized. X-ray diffraction data were collected to 1.75 Å resolution.

Cloning, purification and preliminary X-ray crystallographic analysis of the OmpA-like domain of peptidoglycan-associated lipoprotein from Acinetobacter baumannii

The crystallization of the OmpA-like domain of peptidoglycan-associated lipoprotein from A. baumannii is described.

Cloning, purification and preliminary X-ray data analysis of the human ID2 homodimer

ID2 is an inhibitor of DNA binding that is known to be highly unstable. This study describes the cloning, expression and purification of ID2 for crystallization, culminating in the collection of diffraction data.

Production and crystallization of a panel of structure-based mutants of the human myelin peripheral membrane protein P2

The myelin protein P2 is a peripheral membrane protein functional in lipid bilayer binding and stacking. In order to study the fine details of P2 structure and function, 14 point mutants of human P2 were generated and crystallized; a total of eight different crystal forms were obtained, some of which diffracted to atomic resolution.

Crystallization, X-ray diffraction analysis and SIRAS phasing of human α-L-iduronidase

Native and Hg-derivative diffraction data from human α-L-iduronidase crystals were collected to 2.3 and 3.1 Å resolution, respectively. SIRAS phasing gave a high-quality electron-density map.

Crystallization and X-ray diffraction studies of crustacean proliferating cell nuclear antigen

Proliferating cell nuclear antigen from Litopenaeus vannamei was recombinantly expressed, purified and crystallized. Diffraction data were obtained and processed to 3 Å.

Purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies of catfish (Clarias magur) haemoglobin

Purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies have been carried out on Clarias magur haemoglobin.

Characterization, crystallization and preliminary X-ray diffraction analysis of an (S)-specific esterase (pfEstA) from Pseudomonas fluorescens KCTC 1767: enantioselectivity for potential industrial applications

An enantioselective esterase (pfEstA) from P. fluorescens KCTC 1767 was crystallized in space group P2₁2₁2₁ and diffraction data were collected to a resolution of 1.9 Å.

Crystallization, optimization and preliminary X-ray characterization of a metal-dependent PI-PLC from Streptomyces antibioticus

Crystallization and diffraction analysis of a Ca²⁺-dependent PI-PLC from Streptomyces is reported. Optimization of crystals was completed using a drop-pinning technique and heavy-atom soaks to achieve high-quality diffraction to 1.23 Å.

Crystallization of uridine phosphorylase from Shewanella oneidensis MR-1 in the laboratory and under microgravity and preliminary X-ray diffraction analysis

High-quality crystals of uridine phosphorylase from Shewanella oneidensis were grown under microgravity conditions. X-ray diffraction data were collected to a resolution of 0.95 Å.

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analyses of threonyl-tRNA synthetase editing domain from Aeropyrum pernix

The editing domain of threonyl-tRNA synthetase from the archaeon Aeropyrum pernix has been overexpressed, purified and crystallized. The crystal diffracted to a resolution of 1.66 Å.

Expression, purification, crystallization and preliminary X-ray structure analysis of Vibrio cholerae uridine phosphorylase in complex with thymidine

The expression and purification of uridine phosphorylase from V. cholerae and the crystallization and preliminary X-ray structure analysis of the complex of this enzyme with thymidine are reported.

Crystallization and diffraction of an Isl1–Ldb1 complex

A complex comprising the LIM domains of the LIM-homeodomain protein Isl1 tethered to a peptide region of Ldb1 has been engineered, purified and crystallized. Crystals of this intramolecular complex diffracted to 3.10 Å resolution.

Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501

The successful purification, crystallization and first data collection to 1.8 Å resolution of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501 are reported.

Purification, crystallization and preliminary X-ray diffraction studies of the ATP-binding subunit of an ABC transporter from Geobacillus kaustophilus

An ABC transporter from G. kaustophilus has been crystallized in space group I222. X-ray diffraction data have been collected to 1.60 Å resolution.

Ellman's reagent in promoting crystallization and structure determination of Anabaena CcbP

Ellman's reagent oxidized the free sulfhydryl group of cysteine in Anabaena CcbP protein, facilitating its crystallization.

Dame Louise Napier Johnson (1940–2012)

Obituary for Dame Louise Napier Johnson.

Crystallization and preliminary X-ray diffraction analysis of the high molecular weight keto­acyl reductase FabG4 complexed with NADH. Corrigendum

A corrigendum to the article by Dutta et al. [(2012) Acta Cryst. F68, 786–789].

Crystallization and preliminary X-ray crystallographic analysis of an ice-binding protein (FfIBP) from Flavobacterium frigoris PS1. Addendum

An addendum to the article by Do et al. [(2012) Acta Cryst. F68, 806–809].

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of aspartyl aminopeptidase from the apeB gene of Pseudomonas aeruginosa. Retraction

The article by Natarajan & Mathews [(2012) (2012) Acta Cryst. F68, 207–210] is retracted.