Structure of the hypothetical DUF1811-family protein GK0453 from Geobacillus kaustophilus HTA426

The gene encoding the hypothetical DUF1811-family protein GK0453 from G. kaustophilus was cloned and expressed. The crystal structure of the protein was determined by the molecular-replacement method and was refined to 2.2 Å resolution.

Organo­phosphorus acid anhydrolase from Alteromonas macleodii: structural study and functional relationship to prolidases

The structural study of a bacterial anhydrolase/dipeptidase closely related to human prolidase is reported.

Crystallization and X-ray structure determination of a thermoalkalophilic lipase from Geobacillus SBS-4S

Crystallization and the structure determination at 1.6 Å resolution of LIPSBS, a thermoalkalophilic lipase from Geobacillus strain SBS-4S, are described. The crystals belonged to orthorhombic space group P2₁2₁2₁.

Structure of glutaminyl cyclase from Drosophila melanogaster in space group I4

The structure of ligand-free glutaminyl cyclase from D. melanogaster has been determined in a novel crystal form belonging to space group I4.

Structure of Neisseria meningitidis lipoprotein GNA1162

The crystal structure of GNA1162 from N. meningitidis has been determined at 1.89 Å resolution and its structural analysis provides important information on its function.

Investigating the active centre of the Scytalidium thermophilum catalase

S. thermophilum produces a haem-containing catalase with phenol oxidase activity which is involved in H₂O₂ degradation and the oxidation of some diphenolic compounds in the absence of H₂O₂. The dimensions or volume of the main channel are critical in determining the rate of both movement of H₂O₂ into the active site and dioxygen binding, as has previously been proposed for other monofunctional catalases.

The 1.58 Å resolution structure of the DNA-binding domain of bacteriophage SF6 small terminase provides new hints on DNA binding

The crystal structure of the bacteriophage SF6 small terminase DNA-binding domain has been solved at 1.58 Å resolution. A model for the interaction of G1P with the DNA is proposed.

Structure of a filament of stacked octamers of human DMC1 recombinase

Octameric rings of DMC1 stacked into filaments in the crystal. Similar DMC1–DNA filaments have been observed previously using electron microscopy.

Structure of PCNA from Drosophila melanogaster

Proliferating cell nuclear antigen (PCNA) plays essential roles in DNA replication, DNA repair, cell-cycle regulation and chromatin metabolism. The PCNA from Drosophila melanogaster (DmPCNA) has been purified and crystallized.

Deer mouse hemoglobin exhibits a lowered oxygen affinity owing to mobility of the E helix

The deer mouse, Peromyscus maniculatus, exhibits altitude-associated variation in hemoglobin oxygen affinity. To examine the structural basis of this functional variation, the structure of the hemoglobin has been solved.

Structure of an atypical FeoB G-domain reveals a putative domain-swapped dimer

FeoB is a transmembrane protein involved in ferrous iron uptake in prokaryotic organisms. Here, the structure of the cytoplasmic domain of FeoB from Gallionella capsiferriformans is reported.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50

SAV0479 protein from methicillin- and vancomycin-resistant S. aureus Mu50 was overexpressed and preliminary crystallographic analysis was performed. Diffraction data were collected to a resolution of 2.8 Å. Two Hiramatsu et al. (1997) references, Hiramatsu, Aritaka et al. and Hiramatsu, Hanaki et al. Please indicate in text which is meant

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis

The tryparedoxin peroxidase from L. braziliensis was crystallized and diffraction data were collected to a maximum resolution of 3.3 Å.

Crystallization and preliminary crystallographic analysis of the first condensation domain of viomycin synthetase

The expression, purification and crystallization of the first condensation domain of viomycin synthetase are reported. An initial data set from these crystals extends to 2.9 Å resolution.

Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata

Oligomers of the haemolytic lectin CEL-III from the marine invertebrate C. echinata were crystallized and data were collected to 3.3 Å resolution using synchrotron radiation.

Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase–RNA complex

A complex of the tRNA-modifying enzyme 4-thiouridine synthetase and RNA was crystallized.

Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14

A DING protein from P. aeruginosa strain PA14, named PA14DING or LapC, has been crystallized. The crystals belonged to the monoclinic space group P2₁ and diffracted to 1.9 Å resolution.

Crystallization and preliminary crystallographic analysis of Axe2, an acetylxylan esterase from Geobacillus stearothermophilus

The serine acetylxylan esterase from G. stearothermophilus (Axe2) has been crystallized in the tetragonal space group I422. Complete diffraction data sets have been measured for the selenomethionine derivative (SAD data, 1.70 Å resolution) and the wild-type enzyme (1.85 Å resolution) to be used for a full three-dimensional structural analysis of the Axe2 protein.

Crystallization and preliminary X-ray crystallographic studies of the CARD domain of human CARMA1

The CARD domain of human CARMA1 was crystallized. The crystals belonged to space group P2₁2₁2₁, with unit-cell parameters a = 45.73, b = 53.37, c = 91.89 Å. The crystals were obtained at 293 K and diffracted to a resolution of 3.2 Å.

Crystallization and preliminary X-ray diffraction analysis of the secreted protein Athe_0614 from Caldicellulosiruptor bescii

The Athe_0614 protein secreted by the anaerobic, extremely thermophilic and cellulolytic bacterium C. bescii was crystallized. A diffraction data set was collected to 2.7 Å resolution.

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the N-terminal domain of serine glutamate repeat A (SgrA) protein from Enterococcus faecium

The putative ligand-binding region of serine glutamate repeat A (SgrA) protein from E. faecium was overexpressed, purified and crystallized, and its preliminary X-ray diffraction analysis is reported at 3.3 Å resolution.

Sample preparation, crystallization and structure solution of HisC from Mycobacterium tuberculosis

HisC from M. tuberculosis was overexpressed in M. smegmatis, purified and crystallized, and its structure was solved.

Preliminary crystallographic analysis of the kinase domain of SAD-1, a protein essential for presynaptic differentiation in Caenorhabditis elegans

The kinase domain of SAD-1 from C. elegans has been overexpressed in Escherichia coli BL21 (DE3) cells and purified to homogeneity using nickel–nitrilotriacetic acid metal-affinity, ion-exchange and gel-filtration chromatography. Diffraction-quality crystals were grown using the sitting-drop vapour-diffusion technique.

Purification, crystallization and preliminary X-ray analysis of TP0435 (Tp17) from the syphilis spirochete Treponema pallidum

The soluble portion of TP0435, a putative periplasmic lipoprotein from the syphilis spirochete T. pallidum, has been purified and crystallized in a rhombohedral space group. A complete native data set has been collected to 2.4 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of tannase from Lactobacillus plantarum

A recombinant form of tannase from L. plantarum was purified and crystallized. A native data set has been collected to 1.65 Å resolution.

Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein MoHrip1 from Magnaporthe oryzae

The expression, purification, crystallization and data collection and processing of the effector protein MoHrip1 from M. oryzae are reported.

Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae

A novel effector protein was recombinantly expressed, purified and crystallized. The structure was solved using the selenium SAD method.

Crystallization and preliminary X-ray crystallographic studies of the coiled-coil domain of PIST

The coiled-coil domain of human PIST was crystallized at 293 K and diffracted to a resolution of 4.0 Å. The crystals were found to belong to space group P6₂22 or P6₄22, with unit-cell parameters a = b = 85.19, c = 240.09 Å, γ = 120.00°.