Acta Crystallographica Section F: Structural Biology Communications

Editorial.

Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase

The crystal structure of the psychrotolerant endoclucanase RBcel1, an enzyme implicated in bacterial cellulose synthesis, has been determined to 1.4 Å resolution.

Structure of di­hydrouridine synthase C (DusC) from Escherichia coli

The crystal structure of DusC from E. coli was determined at 2.1 Å resolution.

Structure of monomeric Na-GST-3, a gluta­thione S-transferase from the major human hookworm parasite Necator americanus

The crystal structure of Na-GST-3 from the human hookworm parasite N. americanus is reported.

The structure of substrate-free 1,5-anhydro-D-fructose reductase from Sinorhizobium meliloti 1021 reveals an open enzyme conformation

The crystal structure of 1,5-anhydro-D-fructose reductase from S. meliloti has been determined to 1.93 Å resolution. It is compared with that of 1,5-anhydro-D-fructose reductase from S. morelense and the significance of the observed open conformation of the active site is discussed.

The binding of zinc ions to Emericella nidulans endo-β-1,4-galactanase is essential for crystal formation

Endo-β-1,4-galactanase from E. nidulans crystallized in the presence of 0.2 M zinc acetate contains 15 zinc ions.

Structure of the caspase-recruitment domain from a zebrafish guanylate-binding protein

The crystal structure of the first zebrafish caspase-recruitment domain at 1.47 Å resolution illustrates a six-helix bundle fold similar to that of the human NLRP1 CARD.

Preliminary crystallographic analysis of neuraminidase N2 from a new influenza A virus

The preliminary crystallographic analysis of neuraminidase N2 from influenza virus A/Myanmar/M187/2007 is reported. The crystals belonged to space group P2₁2₁2₁, with four monomers per asymmetric unit.

Crystallization and preliminary X-ray diffraction analysis of selenophosphate synthetases from Trypanosoma brucei and Leishmania major

Selenophosphate synthetases from L. major and T. brucei were crystallized for the first time using limited proteolysis and microbatch techniques and suitable crystals for X-ray diffraction experiments were obtained.

Preliminary X-ray crystallographic study of staphylococcal α-haemolysin monomer

The staphylococcal α-haemolysin H35A mutant has been cloned, expressed and crystallized. The crystals belonged to space group P6₁, with unit-cell parameters a = b = 151.3, c = 145.0 Å.

Protein preparation, crystallization and preliminary X-ray analysis of Polygonum cuspidatum bifunctional chalcone synthase/benzalacetone synthase

P. cuspidatum bifunctional chalcone synthase/benzalacetone synthase was crystallized in the presence of the product and diffraction data were collected to 2.0 Å resolution.

The putative small terminase from the thermophilic dsDNA bacteriophage G20C is a nine-subunit oligomer

The putative small terminase protein from the thermostable bacteriophage G20C has been produced, purified and crystallized.

Crystallization and preliminary X-ray diffraction studies of cyanuric acid hydrolase from Azorhizobium caulinodans

Cyanuric acid hydrolase has been crystallized with the bound inhibitor barbituric acid and diffraction data were collected to 2.7 Å resolution.

Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli

Spermidine acetyltransferase (SAT) from E. coli catalyses the transfer of acetyl groups from acetyl-CoA to spermidine. SAT was crystallized and the crystals contained four molecules in the asymmetric unit.

Expression, purification and preliminary X-ray crystallographic analysis of nitroalkane oxidase (NAO) from Pseudomonas aeruginosa

Nitroalkane oxidase from P. aeruginosa was purified and crystallized. A complete data set was collected to 1.9 Å resolution.

Preliminary X-ray crystallographic analysis of β-carbonic anhydrase psCA3 from Pseudomonas aeruginosa

Two crystal forms of β-carbonic anhydrase psCA3 from P. aeruginosa were grown. Crystal form A belonged to space group P2₁2₁2₁, with unit-cell parameters a = 81.9, b = 84.9, c = 124.2 Å, and diffracted X-rays to 2.9 Å resolution; crystal form B belonged to space group P2₁2₁2, with unit-cell parameters a = 69.9, b = 77.7, c = 88.5 Å, and diffracted X-rays to 3.0 Å resolution.

Expression, purification, crystallization and preliminary crystallographic study of FtsA from methicillin-resistant Staphylococcus aureus

The bacterial cell-division protein FtsA from methicillin-resistant S. aureus was crystallized and X-ray diffraction data were collected to a resolution of 2.3 Å.

Crystallization and preliminary X-ray diffraction analysis of the α subdomain of Lon protease from Brevibacillus thermoruber

The α subdomain of Lon protease from B. thermoruber WR-249 has been expressed, purified and crystallized. Preliminary X-ray diffraction experiments have been undertaken.

Crystallization and preliminary X-ray crystallographic analysis of MxaJ, a component of the methanol-oxidizing system operon from the marine bacterium Methylophaga aminisulfidivorans MP^T

The crystallization and preliminary X-ray crystallographic analysis of the MxaJ protein of the mox operon from the marine bacterium M. aminisulfidivorans MP^T is reported.

Crystallization and preliminary X-ray characterization of the tetra­pyrrole-biosynthetic enzyme porphobilinogen deaminase from Bacillus megaterium

The enzyme porphobilinogen deaminase (PBGD; hydroxymethylbilane synthase; EC 2.5.1.61) catalyses a key early step in the biosynthesis of tetrapyrroles in which four molecules of the monopyrrole porphobilinogen are condensed to form a linear tetrapyrrole. PBGD from B. megaterium was expressed and the enzyme was crystallized in a form which diffracts synchrotron radiation to high resolution.

Expression, purification, crystallization and preliminary X-ray analysis of the extracellular sensory domain of DraK histidine kinase from Streptomyces coelicolor

The expression and crystallization of the extracellular sensory domain of DraK are reported.

Cloning, expression, purification and preliminary X-ray diffraction studies of a novel AB₅ toxin

The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of a novel AB₅ toxin EcxAB are described.

Preliminary X-ray crystallographic studies of the short form of the human TRAF4 TRAF domain

The human TRAF4 TRAF domain was crystallized. The crystals were found to belong to the hexagonal space group P3₂, with unit-cell parameters a = b = 147.17, c = 202.69 Å. The crystals were obtained at 293 K and diffracted to a resolution of 4.2 Å.

Purification, characterization and preliminary X-ray diffraction analysis of a cold-active lipase (CpsLip) from the psychrophilic bacterium Colwellia psychrerythraea 34H

A putative lipase (CpsLip) from C. psychrerythraea strain 34H was expressed, purified and characterized. Crystallization and preliminary X-ray crystallographic analysis of CpsLip were performed.

Cloning, overexpression, crystallization and preliminary X-ray crystallographic analysis of a slow-processing mutant of penicillin G acylase from Kluyvera citrophila

The pac gene encoding penicillin G acylase from K. citrophila was cloned and a slow-processing site-directed mutant was prepared, expressed, purified and crystallized. Triclinic and monoclinic crystal forms were obtained which diffracted to 2.5 and 3.5 Å resolution, respectively.

Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila

Crystals of the β-pore-forming toxin monalysin from the Drosophila pathogen P. entomophila grown from PEG solutions were monoclinic (space group C2) and diffracted to 2.85 Å resolution.

Crystallization and preliminary X-ray analysis of an alanine de­hydrogenase from Bacillus megaterium WSH-002

An alanine dehydrogenase from B. megaterium WSH-002 was expressed in E. coli and purified. Crystallization and preliminary X-ray crystallographic analysis of the recombinant enzyme were performed.

Purification, crystallization and preliminary crystallographic analysis of soybean mature glycinin A1bB2

Soybean mature glycinin was purified and crystallized and its preliminary crystallographic analysis is also reported.

Expression, purification, crystallization and preliminary crystallographic analysis of hepatitis B virus core protein dimerized via a peptide linker containing an EGFP insertion

Hepatitis B virus-like particles consisting of core protein dimers fused to EGFP were expressed, purified and crystallized. The crystals diffracted to 2.8 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040

B. circulans T-3040 cycloisomaltooligosaccharide glucanotransferase was overexpressed in E. coli in two forms and crystallized by the sitting-drop vapour-diffusion method.