issue contents

ISSN: 2053-230X

May 2016 issue

Highlighted illustration

Cover illustration: RRM domain of human RBM7 (Sofos et al., p. 397). In eukaryotic cells, RNA surveillance, maturation and decay depend to a great extent on the 3'-5' exonucleolytic and endonucleolytic activities of the RNA exosome complex. The RRM-protein RBM7 is part of the nuclear exosome targeting (NEXT) complex that directs the nuclear RNA exosome to many of its nucleoplasmic substrates.

research communications

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Two high-resolution co-crystal structures of the mitotic protein kinase haspin with previously reported conjugates consisting of an ATP analogue and a histone H3(1–7) peptide analogue confirm the bisubstrate character of the compounds and provide directions for the future development of more specific haspin inhibitors.

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The expression, purification and crystallization of the DNA-binding Myb domain of LUX ARRHYTHMO from A. thaliana in complex with DNA are reported. The binding affinity of the Myb domain for DNA was measured and was found to be in the low-nanomolar range, suggesting that a single Myb domain is able to bind to its cognate DNA with very high affinity.

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The crystal structure of the dioxygenase FrbJ is reported. An access tunnel was found at the back of the active site, which connects the putative binding site for α-ketoglutarate to the solvent.

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The crystal structure of ketopantoate reductase complexed with NADP+ was determined at 2.3 Å resolution.

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Bile salt hydrolase is an enzyme that is produced by various commensal bacteria in the intestine and is also a key mechanistic microbiome target for enhanced animal and human health.

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The periplasmic sensing domain of V. fischeri chemoreceptor protein A was purified and crystallized. Analysis of an X-ray diffraction data set collected to 1.8 Å resolution is presented.

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The X-ray crystal structure of the RRM domain of human RBM7 reveals a pentameric assembly.

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PvdN (PA2394) from P. aeruginosa is a nonribosomal peptide synthetase tailoring enzyme that is involved in pyoverdine biosynthesis. PvdN has been identified as a periplasmic protein that is pyridoxal 5′-phosphate-dependent and shows homology to class I aminotransferases. Here, the crystal structure of PvdN is reported at 1.22 Å resolution.

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The crystal structure of Prp28 from C. thermophilum is reported at 3.2 Å resolution. This DEAD-box helicase exhibits multiple functions during spliceosome assembly.
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