Structural insights into the catalytic reaction trigger and inhibition of D-3-hydroxybutyrate de­hydrogenase

Crystal structures of A. faecalis D-3-hydroxybutyrate dehydrogenase in complex with NAD⁺ and D-3-hydroxybutyrate, malonate or methylmalonate reveal the mechanisms of the catalytic reaction and its inhibition.

Crystal structure of the cyan fluorescent protein Cerulean-S175G

The crystal structure of Cerulean-S175G was compared with those of Cerulean and SCFP3A, which are notable variants of enhanced cyan fluorescent protein (ECFP). A detailed comparison of the three structures revealed that the notable conformational changes of ECFP variants can be understood mainly in terms of the interaction between the Trp66 residue of the chromophore and residues 145–148 of β-strand 7.

Crystal structure of truncated aspartate transcarbamoylase from Plasmodium falciparum

The crystal structure and preliminary structure-based mutagenesis and activity studies of aspartate transcarbamoylase from P. falciparum are reported.

Crystal structure of a thiolase from Escherichia coli at 1.8 Å resolution

Thiolases catalyze the Claisen condensation of two acetyl-CoA molecules to give acetoacetyl-CoA, as well as the reverse degradative reaction. The present work reveals various structural aspects of E. coli thiolase, including enzyme kinetics, substrate binding, tetrameric organization and the active-site water network.

Structure of a double hexamer of the Pyrococcus furiosus minichromosome maintenance protein N-terminal domain

The structure of a double hexamer of the N-terminal domain of the minichromosome maintenance protein from P. furiosus is presented and analyzed.

LCP crystallization and X-ray diffraction analysis of VcmN, a MATE transporter from Vibrio cholerae

A V. cholerae MATE transporter was crystallized using the lipidic cubic phase (LCP) method. X-ray diffraction data sets were collected from single crystals obtained in a sandwich plate and a sitting-drop plate to resolutions of 2.5 and 2.2 Å, respectively.

Exogenous acetate ion reaches the type II copper centre in CueO through the water-excretion channel and potentially affects the enzymatic activity

The acetate-bound form of copper was found in the crystal structure of the multicopper oxidase CueO. The exogenous acetate ion can reach the copper active site through the water channel in the CueO molecule.

The galactoside 2-α-L-fu­cosyltransferase FUT1 from Arabidopsis thaliana: crystallization and experimental MAD phasing

The crystallization, data collection and experimental phasing using the multiwavelength anomalous dispersion technique of A. thaliana FUT1 is described.

Crystallographic analysis of a subcomplex of the transsulfursome with tRNA for Cys-tRNA^Cys synthesis

A subcomplex of the transsulfursome with tRNA^Cys (SepCysS–SepCysE–tRNA^Cys) represents the second reaction step of Cys-tRNA^Cys synthesis in an indirect pathway. Diffraction-quality crystals (2.6 Å resolution) of this complex were obtained. X-ray crystallographic analysis showed that the complex consists of a SepCysS dimer, a SepCysE dimer and one tRNA^Cys.

Cryoannealing-induced space-group transition of crystals of the carbonic anhydrase psCA3

Crystals of psCA3 undergo a space-group transition from P2₁2₁2 to I222 following cryoannealing, resulting in higher resolution diffraction.