Crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from Helicobacter pylori

The crystal structure of the N-terminal anticodon-binding domain of the nondiscriminating aspartyl-tRNA synthetase from the human pathogen H. pylori was determined at a resolution of 2.0 Å. Two important turns (₇₈GAGL₈₁ and ₈₃NPKL₈₆), along with the conserved Pro84, are proposed to play a crucial role in the recognition of tRNA^Asp and tRNA^Asn.

Crystallization of a fungal lytic polysaccharide monooxygenase expressed from glycoengineered Pichia pastoris for X-ray and neutron diffraction

Polysaccharide monooxygenase 2 from N. crassa was expressed from a glycoengineered strain of P. pastoris. Crystals of the protein bearing modified N-linked glycans showed improved X-ray diffraction at 100 K (1.20 Å resolution) and permitted room-temperature neutron diffraction data collection to 2.12 Å resolution.

Structure of Methylobacterium extorquens malyl-CoA lyase: CoA-substrate binding correlates with domain shift

The structure of M. extorquens malyl-CoA lyase is reported, along with a detailed analysis of the domain motions involved in regulating its enzymatic activity.

Crystallization and X-ray analysis of D-threonine aldolase from Chlamydomonas reinhardtii

Recombinant D-threonine aldolase from the green alga C. reinhardtii was purified to homogeneity and crystallized. An X-ray diffraction data set was collected to 1.85 Å resolution.

Expression and crystallographic studies of D-glycero-β-D-manno-heptose-1-phosphate adenylyltransferase from Burkholderia pseudomallei

D-glycero-β-D-manno-Heptose-1-phosphate adenylyltransferase (HldC) from B. pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data from a selenomethionine-substituted HldC crystal were collected to 2.8 Å resolution.

A natural, single-residue substitution yields a less active peptaibiotic: the structure of bergofungin A at atomic resolution

The crystal structure of the 15-residue peptaibol bergofungin A resembles that of the closely related samarosporin I. The differences in these structures, as well as the significant variation in their respective antibiotic functions, are presented.

The crystal structure of PD1, a Haemophilus surface fibril domain

The crystal structure of PD1 from the Haemophilus surface fibril was determined at a resolution of 3.3 Å, revealing a novel domain arrangement.

Crystallization and X-ray crystallographic analysis of recombinant TylP, a putative γ-butyrolactone receptor protein from Streptomyces fradiae

Recombinant TylP protein from S. fradiae was overproduced, purified to homogeneity and crystallized for X-ray analysis.