The Pex4p–Pex22p complex from Hansenula polymorpha: biophysical analysis, crystallization and X-ray diffraction characterization

The biophysical characterization and crystallization of the Pex4–Pex22 complex from H. polymorpha are reported.

Human CCL5 trimer: expression, purification and initial crystallographic studies

In order to study the oligomeric structure of human CCL5, a new strategy to prepare the CCL5 trimer was developed and a crystal was obtained that diffracted to a satisfactory resolution of 1.87 Å.

X-ray crystallographic and high-speed AFM studies of peroxiredoxin 1 from Chlamydomonas reinhardtii

The oligomeric state of the 2-Cys peroxiredoxin from the green alga Chlamydomonas reinhardtii was determined by an X-ray crystallographic study and high-speed AFM image analysis.

Structure of bovine cytochrome c oxidase in the ligand-free reduced state at neutral pH

Although the enzymatic activity of cytochrome c oxidase (CcO) depends sensitively on pH over a wide range, X-ray structural analyses of bovine CcO have been conducted using crystals prepared at pH 5.7 owing to the difficulty in crystallizing this protein. Here, the structure of ligand-free reduced CcO was successfully determined at 1.99 Å resolution.

Ribokinase from Leishmania donovani: purification, characterization and X-ray crystallographic analysis

Ribokinase from L. donovani was cloned, purified, characterized and crystallized, and data were collected to a resolution of 1.95 Å. X-ray crystallographic analysis showed the presence of two molecules in the asymmetric unit, and human ribokinase could be used as a template to determine the three-dimensional structure.

Structural characterization of Porphyromonas gingivalis enoyl-ACP reductase II (FabK)

The structure of Porphyromonas gingivalis enoyl-ACP reductase II (FabK) was determined via X-ray crystallography to 1.9 Å resolution with all flexible loops visible, FMN fully resolved and the NADPH cofactor partially resolved. Characterization includes the structural basis of NADPH dependence and the role of a required monovalent cation.

Xylanase B from Clostridium cellulovorans 743B: overexpression, purification, crystallization and X-ray diffraction analysis

Cellulosomes are capable of efficiently degrading cellulosic biomass. Crystallization and diffraction analysis of xylanase, which is part of the multi-enzyme cellulosome of Clostridium cellulovorans 743B, are reported.

A thermostable and alkaline GDSL-motif esterase from Bacillus sp. K91: crystallization and X-ray crystallographic analysis

Purification, crystallization and X-ray crystallographic analysis were employed to determine the catalytic mechanism of Est8, a GDSL-motif esterase from Bacillus sp. K91.