High-resolution structure of the Influenza A virus PB2cap binding domain illuminates the changes induced by ligand binding

A crystallographic structure of native influenza A PB2cap was solved to 1.52 Å resolution. B-­factor difference plots and r.m.s.d. plots among known structures suggest functionally relevant disassociation of the N-terminal ligand-binding domain from the C-terminal domain.

YopT domain of the PfhB2 toxin from Pasteurella multocida: protein expression, characterization, crystallization and crystallographic analysis

The crystallization of the YopT-like predicted cysteine protease domain of the secreted toxin PfhB2 from Pasteurella multocida (PfhB2-YopT) is reported. Sequence alignment with structures deposited in the Protein Data Bank indicated no reported homologous structures, suggesting that PfhB2-YopT may represent a novel putative cysteine protease.

The first crystal structure of manganese superoxide dismutase from the genus Staphylococcus

This paper is the first to describe the crystal structure of manganese superoxide dismutase from the genus Staphylococcus. Staphylococcus equorum and Bacillus subtilis MnSODs display different thermal and chemical stabilities. The slightly different structure of S. equorum MnSOD may provide an explanation for the differences in stability.

Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor

The HIV integrase-binding domain (IBD) of lens epithelium-derived growth factor has been cloned, purified and crystallized, and its structure has been solved. IBD forms an unusual domain-swapped dimer that assembles into octamers in the crystal.

Enhancement of the thermostability of mouse claudin-3 on complex formation with the carboxyl-terminal region of Clostridium perfringens enterotoxin improves crystal quality

Mouse claudin-3 was crystallized in complex with the carboxyl-terminal region of C. perfringens enterotoxin and a data set was collected to 4.2 Å resolution.

A novel inhibitor stabilizes the inactive conformation of MAPK-interacting kinase 1

The structure of MAPK-interacting kinase 1 (Mnk1) in complex with a novel Mnk1-selective inhibitor was determined. It was found that the inhibitor stabilizes the auto-inhibited inactive state of Mnk1.

Expression, purification and crystallization of phosphoribosyl transferase from a mycobacteriophage

A putative phosphoribosyl transferase from phage BTCU-1 that infects Mycobacterium and shows no sequence similarity to any structure deposited in the Protein Data Bank was cloned, expressed, purified and crystallized. The crystals diffracted X-rays to 2.2 Å resolution.

Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis

This article reports the crystal structure of MSMEG_4306, a hypothetical protein from Mycobacterium smegmatis, determined by zinc SAD phasing.

Crystal structure of the Kelch domain of human NS1-binding protein at 1.98 Å resolution

NS1-binding protein (NS1-BP) is involved in many cell functions, including pre-mRNA splicing, the ERK signalling pathway, the aryl hydrocarbon receptor pathway, F-actin organization and protein ubiquitylation. Here, the structure of the C-terminal Kelch domain of NS1-BP is reported at 1.98 Å resolution.

Crystal structure of an inferred ancestral bacterial pyruvate de­carboxylase

An ancestral bacterial pyruvate decarboxylase (with an inferred age of 1248 million years) was reconstructed through ancestral sequence reconstruction, synthesized and recombinantly expressed in E. coli. The enzyme is fully functional and its crystal structure was elucidated to 3.5 Å resolution.