Structural characterization of a prolyl aminodipeptidase (PepX) from Lactobacillus helveticus

The crystal structure of the Lactobacillus helveticus PepX enzyme was determined at 2.0 Å resolution, providing the second structural example of a peptidase from the S15 family of the SC clan.

Crystal structure of the Fab region of a neutralizing antibody against granulocyte-macrophage colony-stimulating factor

The crystal structure of the Fab region of a neutralizing fully human antibody against granulocyte-macrophage colony-stimulating factor is reported.

The structure of bovine β-lactoglobulin in crystals grown at pH 3.8 exhibiting novel threefold twinning

Bovine β-lactoglobulin was crystallized at pH 3.8 from 3 M NaCl in a unique crystal form with apparent P6₃22 symmetry. The hexagonal prismatic crystal in fact belonged to space group C222₁, a subgroup of P6₃22, with three molecules in the asymmetric unit and exhibited an unusual type of twinning. The structure was solved by molecular replacement and refined to an R factor of 0.23.

Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization

Rv0100 is an essential acyl carrier protein for the pathogenesis and growth of Mycobacterium tuberculosis. Using Mycobacterium smegmatis as a host, Rv0100 was successfully cloned, purified and crystallized, and the crystals diffracted to 1.9 Å resolution.

Structure of an RNA helix with pyrimidine mismatches and cross-strand stacking

The structure of a 22-base-pair RNA helix with mismatched pyrimidine base pairs is reported. The pyrimidine mismatches are unusual and display sheared, cross-strand stacking geometries that locally constrict the helical width.

Crystal structure of the N-terminal domain of the fibronectin-binding protein PavA from Streptococcus pneumoniae

Pneumococcal adherence and virulence factor A (PavA) plays a key role in the adhesion of Streptococcus pneumoniae to host tissues. Here, the crystal structure of the N-terminal domain of PavA is presented, which reveals structural differences from its homologs.