The RING domain of mitochondrial E3 ubiquitin ligase 1 and its complex with Ube2D2: crystallization and X-ray diffraction

This study presents the crystallization and X-ray diffraction analysis of the RING domain of mitochondrial E3 ubiquitin ligase 1 (MUL1-RING) and its complex with Ube2D2.

The adhesive PitA pilus protein from the early dental plaque colonizer Streptococcus oralis: expression, purification, crystallization and X-ray diffraction analysis

The recombinant production and crystallization of the PitA adhesin from the Streptococcus oralis pilus island 2-encoded sortase-dependent pilus is described. Limited proteolysis of PitA and its complex with terbium crystallophore gave greatly improved X-ray diffraction to 2.16 Å resolution and a sufficiently strong anomalous signal for terbium SAD phasing, respectively.

Crystal structure of an oxidized mutant of human mitochondrial branched-chain aminotransferase

The crystal structure of the oxidized form of a human mitochondrial branched-chain aminotransferase (hBCATm) mutant was determined. The structural analysis supports the concept that a complex regulation mechanism is involved in hBCATm activity, which depends on the key residue Cys315.

Crystallographic analysis of Eisenia hydrolysis-enhancing protein using a long wavelength for native-SAD phasing

Eisenia hydrolysis-enhancing protein from Aplysia kurodai, which is of interest as an indispensable protein for aiding the production of glucose from brown algae, has been purified and crystallized. Native and native-SAD X-ray diffraction data were collected at resolutions of 1.20 and 2.48 Å using wavelengths of 1.0 and 2.1 Å, respectively.

Complexation of the nickel and cobalt transcriptional regulator RcnR with DNA

The bacterial transcriptional regulator of cobalt and nickel efflux, RcnR, has been crystallized with an oligonucleotide encompassing its DNA-binding site.

The Escherichia coli RnlA–RnlB toxin–antitoxin complex: production, characterization and crystallization

A purification protocol for the Escherichia coli RnlA–RnlB toxin–antitoxin complex has been established and the complex was crystallized and characterized using SAXS and native mass spectrometry.

Cocktailed fragment screening by X-ray crystallography of the antibacterial target undecaprenyl pyrophosphate synthase from Acinetobacter baumannii

Acinetobacter baumannii undecaprenyl pyrophosphate synthase, a key enzyme in bacterial cell-wall biosynthesis, has been shown via X-ray structures to bind three fragments identified from direct crystal soaking of a cocktailed screening library.