The crystal structure of the catalytic domain of tau tubulin kinase 2 in complex with a small-molecule inhibitor

The first crystal structure of the kinase domain of tau tubulin kinase 2 in complex with a small-molecule inhibitor is described.

Structural and biochemical characterization of mitochondrial citrate synthase 4 from Arabidopsis thaliana

The crystal structure of mitochondrial citrate synthase 4 from Arabidopsis thaliana was determined at a resolution of 2.69 Å and is the first structure of a plant mitchondrial citrate synthase.

Engineering the Fab fragment of the anti-IgE omalizumab to prevent Fab crystallization and permit IgE-Fc complex crystallization

The omalizumab Fab was engineered to disrupt recurring crystal packing interactions in Fab crystal structures; this led to the eventual structure determination of an omalizumab-derived Fab in complex with its target, IgE-Fc.

Structure determination of the human TRPV1 ankyrin-repeat domain under nonreducing conditions

The structure of the human TRPV1 ankyrin-repeat domain was determined by X-ray crystallography. This structure showed a unique solvent-accessible pocket near Cys258 and a unique conformation of its finger loop 3, the conformation of which is significantly different from that in the rat homologue.

The phospholipase A effector PlaA from Legionella pneumophila: expression, purification and crystallization

The Legionella pneumophila phospholipase named PlaA is a secreted effector that plays an essential role in maintaining the integrity of Legionella-containing vacuoles in L. pneumophila pathogenesis. Crystals of PlaA and its selenomethionine-substituted form were used for X-ray analysis and diffracted to resolutions of 2.2 and 2.7 Å, respectively.

Structural insights into the catalytic mechanism of Bacillus subtilis BacF

Crystal structures of Bacillus subtilis BacF provide an insight into stereospecific substrate recruitment in a fold type I transaminase enzyme.

Crystal structure of Arabidopsis thaliana neutral invertase 2

The structure of neutral invertase 2 from Arabidopsis thaliana was solved at 3.4 Å resolution. This first structure of a GH100 family enzyme from a higher plant reveals a remarkable similarity to that of the neutral invertase InvA from the cyanobacterium Anabaena, pointing to high evolutionary conservation.