issue contents
November 2020 issue
research communications
Crystal structures of the C-domain of P140 from M. genitalium adopt an alternative conformation to that recently found in the intact extracellular region of P140. The plasticity of the C-domain is related to the functioning of the mycoplasma adhesion complex.
The D-amino-acid oxidase from the thermophilic fungus R. emersonii strain YA (ReDAAO) has a high thermal stability and a unique broad substrate specificity. In this study, in order to understand the structural factors that contribute to its unique characteristics, ReDAAO was crystallized and its crystal structure was determined.
PDB reference: D-amino-acid oxidase, 7ct4
Open access
The structure of the class II fructose-1,6-bisphosphatase from F. tularensis is presented together with kinetic data for the wild type and the active-site mutant Thr89Ser. The structure reveals a tetramer of four identical subunits with approximate 222 symmetry. The structure is related to the homologous structure from the related pathogen Mycobacterium tuberculosis.
Serratia marcescens is an opportunistic pathogen that can use chitin-enriched nutrients as an alternative energy source. We identified a chitoporin (ChiP), termed SmChiP, from the outer membrane of S. marcescens and report its crystallization without and with chitohexaose. Initial assessment of the complex structure revealed electron density for the sugar ligand.
L. donovani Rab5a (LdRab5a), a GTP-dependent molecular switch, regulates the fluid-phase endocytosis of essential nutrients. The crystal structure of the GTPase domain of LdRab5a has been determined in the GDP-bound form, which shows the canonical Rab fold but with a unique disposition of the Switch I region.
PDB reference: GDP-bound GTPase domain of Rab5a, 6l6o
Crystal structures of a bacterial nonhydrolyzing UDP-GlcNAc 2-epimerase with and without substrates are described. The structures, when compared with homologous enzymes, shed additional light on the mechanism and understanding of the non-allosteric nature of the enzyme.
methods communications
The successful structure determination of a triclinic form of Dps from Escherichia coli using multi-crystal data-collection techniques is described.
PDB reference: Dps, 6qvx