Crystal structure of the TLDc domain of human NCOA7-AS

The crystal structure of the TLDc domain of the human NCOA7-AS protein, which acts as an interferon-induced antiviral inhibitor, is reported.

LrpCBA pilus proteins of gut-dwelling Ligilactobacillus ruminis: crystallization and X-ray diffraction analysis

Ligilactobacillus ruminis is a strict anaerobic gut bacterium that produces sortase-dependent pili (LrpCBA) consisting of the tip LrpC (∼123 kDa), basal LrpB (∼39 kDa) and backbone LrpA (∼49 kDa) pilin subunits. Here, the strategy used for the recombinant expression, crystallization and X-ray diffraction analysis of all three pilus constituents is reported.

Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB

A structure of the Rhodococcus equi virulence factor VapB in a new crystal form reveals conformational changes in loop regions at the top of the eight-stranded β-barrel. The resulting cavity might form the binding site for a potential ligand.

The identification and structural analysis of potential 14-3-3 interaction sites on the bone regulator protein Schnurri-3

The structures of two peptide motifs of Schnurri-3 in complex with 14-3-3 are reported.

Structures of the Plasmodium falciparum heat-shock protein 70-x ATPase domain in complex with chemical fragments identify conserved and unique binding sites

233 chemical fragments were screened for binding to the ATPase domain of the Hsp70-x chaperone from the malaria parasite Plasmodium falciparum. Crystallographic structures of this domain in complex with different fragments revealed a major binding site proximal to the ATPase catalytic pocket. It is shown that a residue near this binding site is not conserved between P. falciparum and human erythrocytic Hsp70 chaperones, which may provide a basis for specific inhibition of the parasite enzyme.

The X-ray structure of L-threonine de­hydrogenase from the common hospital pathogen Clostridium difficile

The L-threonine dehydrogenase from Clostridium difficile has been cloned, expressed and crystallized, allowing the X-ray structure to be determined at 2.6 Å resolution with an R factor of 20.0% and an R_free of 23.9%.