Crystal structures of HER3 extracellular domain 4 in complex with the designed ankyrin-repeat protein D5

The structure of a designed ankyrin-repeat protein that selectively binds to domain 4 of HER3, an important driver of malignant growth in many tumors, has been determined. The structure helps to explain the selectivity against other members of the HER family, and binding to this epitope will interfere with its interactions in the tethered (inactive) and extended (active) conformations.

Phosphorus and sulfur SAD phasing of the nucleic acid-bound DNA-binding domain of interferon regulatory factor 4

Solution of the structure of the DNA-binding domain of interferon regulatory factor 4 bound to its interferon-stimulated response element by native intrinsic phosphorus and sulfur single-wavelength anomalous dispersion methods (native SAD) is described.

Structure of an H3N2 influenza virus nucleoprotein

The influenza virus nucleoprotein binds to the viral RNA genome and is essential for virus replication. Here, the structure of the nucleoprotein from an H3N2 virus is presented at 2.2 Å resolution.

Crystal structures of Val58Ile tryptophan repressor in a domain-swapped array in the presence and absence of L-tryptophan

In a domain-swapped, gel-like crystalline form of Escherichia coli tryptophan repressor, the physiological ligand L-tryptophan binds equivalently as in the native dimeric repressor, even though the binding-site residues originate from three distinct polypeptide chains instead of two, and large solvent channels accommodate a disordered N-terminal extension. Proteins that cannot otherwise be crystallized might be oriented in the channels for diffraction analysis by exploiting these features.

PAIREF: paired refinement also for Phenix users

Support for the refinement engine phenix.refine has been implemented into PAIREF, a tool providing automatic paired refinement.