Preliminary X-ray diffraction and ligand-binding analyses of the N-terminal domain of hypothetical protein Rv1421 from Mycobacterium tuberculosis H37Rv

A crystal of the N-terminal domain of hypothetical protein Rv1421 from Mycobacterium tuberculosis H37Rv (MtRv1421-NTD) diffracted to 1.7 Å resolution. MtRv1421-NTD, which contains a Walker A/B-like motif, can bind uridine diphosphate (UDP) and UDP-N-acetylglucosamine, indicating that the presence of a UDP moiety on the ligand may be essential for its interaction.

The structure of a pectin-active family 1 polysaccharide lyase from the marine bacterium Pseudoalteromonas fuliginea

The structure of PfPL1, the first structure of a polysaccharide lyase family 1 subfamily 2 enzyme, reveals a parallel β-helix fold. Structural comparisons reveal insights into substrate recognition and key elements in the catalytic machinery.

Structural analysis of the FERM domain of human protein tyrosine phosphatase non-receptor type 21

The crystal structure of the FERM domain of protein tyrosine phosphatase non-receptor type 21, a regulator of cancer progression and invasion, was determined and an atomic-level structural analysis was conducted.

The impact of exchanging the light and heavy chains on the structures of bovine ultralong antibodies

Complementary-determining region 3 (CDR3) of the light chain interacts with the extended CDR3 of the paired heavy chain in bovine ultralong antibodies. Exchanging light chains between two different ultralong antibodies induced a small axial twist in the CDR3H β-ribbon stalk region; however, comparative crystallographic interface analysis indicates that the ultralong loops have flexibility and their positioning is affected by crystal packing.