High-resolution structure of the native histone octamer

The high-resolution (1.90 Å) model of the native histone octamer allows structural comparisons to be made with the nucleosome-core particle, along with an identification of a likely core-histone binding site.

Structure of Citrobacter freundii L-me­thionine γ-lyase

The three-dimensional structure of C. freundii L-methionine γ-lyase has been determined at 1.9 Å resolution.

Purification, crystallization and preliminary diffraction studies of an ectromelia virus glutaredoxin

Ectromelia virus glutaredoxin has been crystallized in the presence of the reducing agent DTT. A diffraction data set has been collected and processed to 1.8 Å resolution.

Overexpression, purification and crystallographic analysis of a unique adenosine kinase from Mycobacterium tuberculosis

Adenosine kinase from M. tuberculosis has been overexpressed, purified and crystallized in the presence of adenosine. Structure determination using molecular replacement with diffraction data collected at 2.2 Å reveals a dimeric structure.

Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8

The production, purification, crystallization and preliminary X-ray crystallographic analysis of adeno-associated virus serotype 8 is reported.

Crystallization and preliminary X-ray diffraction studies of the cysteine protease ervatamin A from Ervatamia coronaria

Ervatamin A is a papain-family cysteine protease with high activity and stability. It has been isolated and purified from the latex of the medicinal flowering plant E. coronaria and crystallized by the vapour-diffusion technique. Crystals diffracted to 2.1 Å and the structure was solved by molecular replacement.

Crystallization of a protein using dehydration without a precipitant

Crystals grown by simple dehydration from a solution of hypoxic response protein I (HRPI) from M. tuberculosis diffract to a maximum resolution of 2.1 Å

Acanthamoeba polyphaga mimivirus NDK: preliminary crystallographic analysis of the first viral nucleoside diphosphate kinase

A. polyphaga mimivirus, the largest known double-stranded DNA virus, is the first virus to exhibit a nucleoside diphosphate kinase gene. The expression and crystallization of the viral NDK are reported.

Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA

Preliminary data analysis of crystals of a designed six-finger zinc-finger protein bound to DNA is presented. The cell likely contains two protein–DNA complexes and diffracts to 2.95 Å.

Crystallization and preliminary X-ray diffraction analysis of the electron-transfer complex between the terminal oxygenase component and ferredoxin in the Rieske non-haem iron oxygenase system carbazole 1,9a-dioxygenase

The electron-transfer complex between the terminal oxygenase and ferredoxin of carbazole 1,9a-dioxygenase was crystallized and diffraction data were collected to 1.90 Å resolution.

A preliminary crystallographic analysis of the putative mevalonate diphosphate de­carboxylase from Trypanosoma brucei

The gene encoding the putative mevalonate diphosphate decarboxylase, an enzyme from the mevalonate pathway of isoprenoid precursor biosynthesis, has been cloned from T. brucei. Recombinant protein has been expressed, purified and highly ordered crystals obtained and characterized to aid the structure–function analysis of this enzyme.

Oligonucleotides with cyclohexene-nucleoside building blocks: crystallization and preliminary X-ray studies of a left-handed sequence GTGTACAC

A novel oligonucleotide containing cyclohexene-nucleoside building blocks has been crystallized. Crystals belong to space group R3 and diffract to 1.7 Å.

Purification, crystallization and preliminary crystallographic analysis of the Hermes transposase

Upon purification, an N-terminally deleted version of the Hermes transposase exists in solution as a mixture of two species that are approximately hexameric and dimeric. Crystals have been obtained of the smaller species that diffract to 2.1 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of a nucleoside kinase from the hyperthermophile Methanocaldococcus jannaschii

Nucleoside kinase from the hyperthermophilic archaeon M. jannaschii is a member of the PFK-B family which belongs to the ribokinase superfamily. Here, its expression, purification, crystallization and preliminary X-ray analysis are described.

Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis

Crystals of the 39 kDa functional form of methylthioribose-1-phosphate isomerase from B. subtilis diffracted to 2.50 Å.

Crystallization and preliminary X-ray analysis of the C-terminal cytoplasmic domain of FlhA, a membrane-protein subunit of the bacterial flagellar type III protein-export apparatus

Crystals of the cytoplasmic domain of FlhA, a membrane-protein component of the bacterial flagellar type III protein-export apparatus from Salmonella, have been obtained and characterized by X-ray diffraction.

Crystallization and preliminary X-ray analysis of molecular chaperone-like diol dehydratase-reactivating factor in ADP-bound and nucleotide-free forms

The molecular chaperone-like reactivating factor for adenosylcobalamin (coenzyme B₁₂) dependent diol dehydratase was crystallized in ADP-bound and nucleotide-free forms. Preliminary X-ray analysis indicated that crystals are orthorhombic and diffract to 2.0 Å.

Crystallization and preliminary crystallographic analysis of an aminoglycoside kinase from Legionella pneumophila

Two crystal forms of the antibiotic resistance enzyme APH(9)-Ia from L. pneumophila are reported.

Crystallization and avoiding the problem of hemihedral twinning in crystals of Δ¹-pyrroline-5-carboxylate de­hydrogenase from Thermus thermophilus

Δ¹-Pyrroline-5-carboxylate dehydrogenase from T. thermophilus has been crystallized in a citrate-bound form. The crystals exhibited hemihedral twinning and untwinned crystals were obtained by exclusion of lithium sulfate from the crystallization solution. Apo, NAD⁺ bound-, NADH bound- and glutamate-bound forms were also obtained and characterized by X-ray diffraction.

Crystallization and preliminary X-ray crystallographic analysis of rat calcineurin B homologous protein 1

Calcineurin B homologous protein 1 from rat was expressed in E. coli, purified and crystallized. A full set of X-ray diffraction data has been collected to 2.2 Å resolution using a synchrotron-radiation source.

Complex assembly, crystallization and preliminary X-ray crystallographic studies of rhesus macaque MHC Mamu-A*01 complexed with an immunodominant SIV-Gag nonapeptide

Crystallization of the first rhesus macaque MHC class I complex.

Crystallization and preliminary X-ray diffraction analysis of the sialic acid-binding domain (VP8*) of porcine rotavirus strain CRW-8

The sialic acid-binding domain (VP8*) component of the porcine CRW-8 rotavirus spike protein has been overexpressed in E. coli, purified and co-crystallized with an N-acetylneuraminic acid derivative. X-ray diffraction data have been collected to 2.3 Å, which has enabled determination of the structure by molecular replacement.