The novel 2Fe–2S outer mitochondrial protein mitoNEET displays conformational flexibility in its N-terminal cytoplasmic tethering domain

The crystal structure of the anti-diabetic drug target mitoNEET obtained from a GFP fusion construct (1.4 Å resolution, R factor = 20.2%) shows that the CDGSH 2Fe–2S binding domains are superimposable with previously determined non-fused constructs. However, there is considerable flexibility in the position of the outer mitochondrial tethering arms resulting in two different conformations in the crystal structure.

Interaction of an echinomycin–DNA complex with manganese ions

In the presence of Mn²⁺, a new crystal form of an echinomycin–d(ACGTACGT) complex is found which shows mixed base pairing next to the bis-intercalation site.

Structure of the lamin A/C R482W mutant responsible for dominant familial partial lipodystrophy (FPLD)

The crystal structure of the R482W mutant of the C-terminal domain of lamin A/C, which is responsible for familial partial lipodystrophy, has been determined at 1.5 Å resolution. A completely novel aggregation state of the mutated protein may be responsible for the changes in biological activity.

Inhibitor design for ribonuclease A: the binding of two 5′-phosphate uridine analogues

The structure of ribonuclease A in complex with uridine 5′-phosphate and uridine 5′-diphosphate has been determined at 1.4 Å resolution in order to facilitate the rational design of selective and potent inhibitors.

Crystallization and preliminary crystallographic analysis of bifunctional γ-glutamylcysteine synthetase–glutatione synthetase from Streptococcus agalactiae

The S. agalactiae bifunctional ligase for glutathione synthesis has been crystallized. Native data were collected to 2.8 Å resolution.

Purification, crystallization and preliminary X-ray analysis of haemoglobin from ostrich (Stru­thio camelus)

Purification, crystallization and preliminary X-ray analysis of haemoglobin from ostrich (Struthio camelus) has been carried out under 293 K temperature conditions. The ostrich is a large flightless bird which contains inositol tetrakisphosphate in erythrocytes and its whole blood oxygen affinity is higher. Efforts have been made to explore the structure–function relationship of ostrich heamoglobin.

Purification, crystallization and preliminary X-ray diffraction analysis of the FeoB G domain from Methanococcus jannaschii

The N-terminal domain of FeoB from Methanococcus jannaschii was overproduced, purified to homogeneity and crystallized in the presence of GTP and magnesium.

Crystallization and preliminary X-ray diffraction studies of the tetramerization domain derived from the human potassium channel Kv1.3

The tetramerization domain of human Kv1.3 was cloned, expressed, purified and crystallized. The crystals belonged to space group I4 and diffracted to 1.2 Å resolution using synchrotron radiation.

Crystallization and preliminary crystallographic studies of the single-chain variable fragment of antibody chA21 in complex with an N-terminal fragment of ErbB2

An antibody–antigen complex consisting of a single-chain variable fragment of the potential therapeutic antibody chA21 and an N-terminal fragment (residues 1–192) of the human ErbB2 extracellular domain was expressed, purified and crystallized. X-ray diffraction data were collected to 2.45 Å resolution.

Crystallization and preliminary X-ray diffraction studies of AsaP1_E294A and AsaP1_E294Q, two inactive mutants of the toxic zinc metallopeptidase AsaP1 from Aeromonas salmonicida subsp. achromogenes

Crystallization and preliminary X-ray diffraction studies of AsaP1_E294A and AsaP1_E294Q, two inactive mutants of the toxic zinc metallopeptidase AsaP1 from A. salmonicida subsp. achromogenes, are reported.

Crystallization and preliminary X-ray characterization of a glycerol de­hydrogenase from the human pathogen Salmonella enterica serovar Typhimurium

The expression, purification and crystallization of a glycerol dehydrogenase from S. typhimurium is decribed. The crystals diffracted to 3.5 Å resolution.

Expression, purification, crystallization and preliminary X-ray studies of Vibrio cholerae pseudopilin EpsH

Recombinant V. cholerae EpsH has been expressed, purified and crystallized. The crystals diffracted to 1.71 Å resolution.

Production, purification, crystallization and preliminary X-ray diffraction analysis of the HIV-2-neutralizing V3 loop-specific Fab fragment 7C8

Neutralizing Fab fragments of the HIV-2-binding murine antibody 7C8 were generated after purification from hybridoma cell-culture supernatant. Crystallization conditions were determined and diffraction data were collected to 2.7 Å resolution.

Protein preparation, crystallization and preliminary crystallographic studies of Bacillus subtilis glycinamide ribonucleotide transformylase

In order to study the structure and function of B. subtilis glycinamide ribonucleotide transformylase, the purN gene was amplified, cloned into an expression vector and expressed in soluble form in Escherichia coli. The protein was purified to homogeneity and crystals suitable for X-ray data collection were obtained.

A crystallizable form of the Streptococcus gordonii surface antigen SspB C-domain obtained by limited proteolysis

The C-terminal domain of the S. gordonii surface protein SspB has been expressed, purified and crystallized. Diffraction data have been collected to 2.1 Å resolution.

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9

The purification, crystallization and preliminary X-ray crystallographic analyses of the AAV9 viral capsid are reported.

Preliminary crystallographic studies of the regulatory domain of response regulator YycF from an essential two-component signal transduction system

The regulatory domain of response regulator YycF from an essential two-component signal transduction system has been crystallized and X-ray data have been collected at 1.95 Å resolution.

Preliminary structural characterization of human SOUL, a haem-binding protein

This manuscript describes the overexpression, purification and crystallization of human SOUL protein (hSOUL). hSOUL is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placenta.

Crystallization and preliminary X-ray crystallographic studies of DesR, a thermosensing response regulator in a two-component signalling system from Streptococcus pneumoniae

The response regulator DesR from S. pneumoniae was cloned, expressed, purified and crystallized. A complete data set was collected to 1.7 Å resolution.

Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas

Diifraction data have been collected for the apo form of the orange protein from D. gigas to 2.25 Å resolution in-house and to beyond 2.0 Å resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 Å.

Crystallization and initial crystallographic analysis of phosphoglucosamine mutase from Bacillus anthracis

The enzyme phosphoglucosamine mutase from B. anthracis participates in the peptidoglycan-biosynthetic pathway. The expression, purification and crystallization of this enzyme are described; diffraction data have been collected to 2.7 Å resolution.

Purification, crystallization and preliminary crystallographic studies of a Kunitz-type proteinase inhibitor from tamarind (Tamarindus indica) seeds

A 21 kDa Kunitz-type proteinase inhibitor was purified from tamarind (T. indica) seeds, crystallized and characterized by X-ray diffraction.

Production, crystallization and preliminary crystallographic analysis of an exosite-containing fragment of human von Willebrand factor-cleaving proteinase ADAMTS13

A fragment of the ADAMTS13 ancillary domains (ADAMTS13-DTCS) has been expressed, purified and crystallized and the crystals have been characterized by X-ray diffraction.

Crystallization and preliminary X-ray crystallographic analysis of Escherichia coli CusB

This article describes how CusB from E. coli was overexpressed and the recombinant protein purified using Ni–NTA affinity, Q anion-exchange and gel-filtration chromatography, and how the purified CusB protein was crystallized using the vapour-diffusion method.