The structure of mAG, a monomeric mutant of the green fluorescent protein Azami-Green, reveals the structural basis of its stable green emission

The crystal structure of a monomeric mutant of Azami-Green (mAG) from G. fascicularis was determined at 2.2 Å resolution.

Structural studies of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv

The first crystal structure of phosphoglucose isomerase from M. tuberculosis H37Rv is reported.

Structure of orotate phosphoribosyltransferase from the caries pathogen Streptococcus mutans

The crystal structure of OPRTase from the caries pathogen Streptococcus mutans is reported at 2.4 Å resolution.

Crystallization and preliminary X-ray studies of azoreductases from Bacillus sp. B29

In order to determine the structure–function relationship of the azo-dye reduction mechanism, an X-ray crystallographic study of azoreductases was performed. Selenomethionine-labelled AzrA (SeMet-AzrA) and AzrC were crystallized by the hanging-drop vapour-diffusion method.

Purification, crystallization and preliminary X-ray analysis of apo glyceraldehyde-3-phosphate de­hydrogenase 1 (GAP1) from methicillin-resistant Staphylococcus aureus (MRSA252)

The purification, crystallization and preliminary X-ray crystallographic analysis of glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) from MRSA252 in the apo form is reported.

Cloning, overexpression, purification, crystallization and preliminary X-ray analysis of a female-specific lipocalin (FLP) expressed in the lacrimal glands of Syrian hamsters

A female-specific lacrimal protein from Syrian hamsters has been crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2₁2₁2₁ and diffraction data were collected to 1.86 Å resolution.

Crystallization and preliminary crystallographic studies of a flavin-dependent thymidylate synthase from Helicobacter pylori

Crystals of C-terminally His-tagged ThyX from H. pylori belonged to space group C2, with unit-cell parameters a = 221.92, b = 49.43, c = 143.02 Å, β = 98.84°, and diffracted to beyond 2.5 Å resolution.

Crystallization and preliminary X-ray analysis of the ligand-binding domain of cAMP receptor protein

N-terminal fragments of the cyclic AMP receptor protein from E. coli created by two different proteases, subtilisin and chymotrypsin, have been crystallized and diffracted to 2.0 and 2.8 Å resolution, respectively.

Crystallization and preliminary X-ray crystallographic analysis of human phosphodiesterase 12

The endonuclease/exonuclease/phosphatase-homology domain of phosphodiesterase PDE12 has been crystallized. The crystals diffracted to 2.5 Å resolution.

Crystallization and preliminary structural analyses of glutamate de­hydrogenase from Peptoniphilus asaccharolyticus

Selenomethionine-derivatized glutamate dehydrogenase from P. asaccharolyticus has been crystallized. Diffraction data were collected to 3.5 Å resolution from crystals belonging to the rhombohedral space group H32 and structure determination is in progress.

Crystallization and preliminary X-ray crystallographic analysis of MinE, the cell-division topological specificity factor from Helicobacter pylori

H. pylori MinE has been expressed, purified and crystallized. Native and MAD data sets have been collected from H. pylori MinE crystals to 2.8 and 3.0 Å resolution, respectively.

Preliminary X-ray crystallographic analysis of SMU.2055 protein from the caries pathogen Streptococcus mutans

The SMU.2055 gene from the major caries pathogen Streptococcus mutans was cloned and native and SeMet-labelled SMU.2055 proteins were expressed at a high level. Diffraction-quality crystals of SeMet-labelled SMU.2055 were obtained using the sitting-drop vapour-diffusion method and diffracted to a resolution of 2.5 Å.

Purification, crystallization and preliminary X-ray diffraction analysis of the lytic transglycosylase MltF from Escherichia coli

Two soluble His-tagged forms of the lytic transglycosylase MltF, one containing full length protein, the other containing its N-terminal domain, have been successfully overexpressed, purified and crystallized. X-ray diffraction data were collected for both crystal forms, including a MAD data set to 2.7 Å for the MltF N-terminal domain.

Crystallization and preliminary X-ray diffraction analysis of a self-complementary DNA heptacosamer with a 20-base-pair duplex flanked by seven-nucleotide overhangs at the 3′-terminus

Triclinic crystals of a self-complementary DNA heptacosamer with 20-base-pair duplexes flanked by 3′-terminal seven-nucleotide overhangs have been obtained. X-ray data were collected to 2.8 Å resolution using synchrotron radiation.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the N-terminal carbohydrate-recognition domain of human galectin-4

The N-terminal carbohydrate-recognition domain of human galectin-4 has been crystallized by the vapour-diffusion technique using polyethylene glycol as the precipitant agent. A complete data set was collected from a native crystal to 2.2 Å resolution.

Crystallization and preliminary crystallographic analysis of the central domain of Drosophila Dribble, a protein that is essential for ribosome biogenesis

A soluble domain spanning amino-acid residues 16–197 of the Drosophila Dribble protein was overexpressed in E. coli, purified and crystallized. X-ray diffraction data were collected to beyond 2 Å resolution.

A preliminary crystallographic study of recombinant NicX, an Fe²⁺-dependent 2,5-di­hydroxypyridine dioxygenase from Pseudomonas putida KT2440

The Fe²⁺-dependent dioxygenase NicX from P. putida KT2440 has been crystallized using the vapour-diffusion method. Native data have been collected to 2.0 Å resolution.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis

The macrophage growth locus A (MglA) protein from F. tularensis crystallized in the hexagonal space group P6₁ or P6₅, with unit-cell parameters a = b = 125, c = 54 Å.

Crystallization and preliminary X-ray diffraction analysis of West Nile virus

Crystals of infectious West Nile virus were obtained and diffracted at best to about 25 Å resolution. Preliminary analysis of the diffraction pattern suggested tight hexagonal packing of the intact virus.

Expression, crystallization and preliminary crystallographic analysis of RNA-binding protein Hfq (YmaH) from Bacillus subtilis in complex with an RNA aptamer

The RNA-binding protein Hfq from B. subtilis was crystallized using the hanging-drop vapour-diffusion method in two crystal forms that belonged to space groups I422 and F222; diffraction data were collected to 2.2 Å resolution from both forms.

Crystallization and preliminary X-ray diffraction analysis of the dimerization domain of the tumour suppressor ING4

The N-terminal domain of ING4 was overexpressed in Escherichia coli and purified to homogeneity. Crystallization experiments yielded crystals that were suitable for high-resolution X-ray diffraction analysis.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of glyoxalase I from Leishmania infantum

Glyoxalase I from L. infantum was cloned, overexpressed in E. coli, purified and crystallized.

Crystallization and preliminary X-ray analysis of 6-hydroxymethyl-7,8-di­hydropterin pyro­phosphokinase from Staphylococcus aureus

The expression, purification and crystallization of S. aureus 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, an essential enzyme from the folate-biosynthesis pathway, is reported.

Crystallization and preliminary crystallographic analysis of nosiheptide-resistance methyltransferase from Streptomyces actuosus in complex with SAM

The expression, purification and crystallization of nosiheptide-resistance methyltransferase (NSR) from Streptomyces actuosus is described.

Crystallization and preliminary structural characterization of the two actin-depolymerization factors of the malaria parasite

The expression, purification and crystallization of Plasmodium actin-depolymerization factors 1 and 2 are described. X-ray diffraction data were collected to 2.0 and 2.1 Å resolution, respectively, and the structures of both proteins in solution were characterized.

Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX

The expression, purification and crystallization of the periplasmic protein AlgX from P. aeruginosa is described. The crystals diffracted to 2.1 Å resolution.

Crystallization and initial X-ray diffraction analysis of a mannose-binding lectin from champedak

Champedak mannose-binding lectin was crystallized at 293 K. Crystallization conditions and preliminary X-ray diffraction analysis are reported.

Dramatic improvement of crystal quality for low-temperature-grown rabbit muscle aldolase

Rabbit muscle aldolase (RMA) was crystallized in complex with the low-complexity domain (LC4) of sorting nexin 9. Monoclinic crystals were obtained at room temperature that displayed large mosaicity and poor X-ray diffraction. However, orthorhombic RMA–LC4 crystals grown at 277 K under similar conditions exhibited low mosaicity, allowing data collection to 2.2 Å Bragg spacing and structure determination.

Expression and crystallization of SeDsbA, SeDsbL and SeSrgA from Salmonella enterica serovar Typhimurium

The cloning, purification, crystallization and preliminary crystallographic studies of three DsbA-like proteins present in S. enterica serovar Typhimurium, SeDsbA, SeDsbL and SeSrgA, are reported.

Cloning, purification, crystallization and X-ray crystallographic analysis of Ignicoccus hospitalis neelaredoxin

Diffraction-quality crystals of I. hospitalis neelaredoxin have been produced. The cloning, expression, purification, crystallization and X-ray crystallographic analysis are reported.

Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum

To determine the binding mechanism of BoNT/OFD05 and its ganglioside receptors on neuronal cells, recombinant BoNT/OFD05 receptor-binding domain has been expressed, purified and crystallized.

Crystallization and preliminary X-ray crystallographic analysis of the tetracycline-degrading monooxygenase TetX2 from Bacteroides thetaiotaomicron

Crystallization and preliminary X-ray crystallographic analysis of the tetracycline-degrading monooxygenase TetX2 from B. thetaiotaomicron are reported.

Trial by fire: are the crystals macromolecules?

A simple `melt test' to distinguish salt crystals from macromolecule crystals is described.