6-Phosphogluconate de­hydrogenase and its crystal structures

6-Phosphogluconate dehydrogenase is in a process of a continuous discovery: despite the determination of several crystal structures over thirty years, a clear elucidation of its mechanism from the structure–function point of view has not yet been reached.

Structural and functional analysis of the SET3 histone de­acetylase complex

An electron-microscopy model of the SET3 histone deacetylase complex reveals a three-lobe architecture.

Structural investigation of a pyrano-1,3-oxazine derivative and the phenanthridinone core moiety against BRD2 bromodomains

BRD2 is a promising drug target for the treatment of various cancers. Crystal structures of BRD2 bromodomains BD1 and BD2 in complexes with a pyrano-1,3-oxazine derivative and phenanthridinone compounds were determined. Crystal structures and biochemical assays confirm that these ligands are indeed potent inhibitors of BRD2 bromodomains.

Conformational flexibility in the zinc solute-binding protein ZnuA

In bacteria, high-affinity zinc import is accomplished by ATP-binding cassette (ABC) transporters, which rely on extracellular solute-binding proteins (SBPs) to acquire the metal and deliver it to the membrane permease. The crystal structure of the zinc-bound (holo) form of Citrobacter koseri SBP ZnuA has been determined. Despite 95% sequence identity to the ZnuA homologue from Salmonella enterica, C. koseri ZnuA exhibits different zinc-coordination and a closed rather than open conformation. Comparison with other close ZnuA homologue structures suggests a flexible conformational landscape that may be important for zinc binding and/or delivery to the permease.

Crystal structure of an inorganic pyrophosphatase from Chlamydia trachomatis D/UW-3/Cx

Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infections. C. trachomatis inorganic pyrophosphatase (CtPPase) hydrolyzes inorganic pyrophosphate during metabolism. A 2.2 Å resolution X-ray structure of CtPPase reveals shared structural features that may facilitate the repurposing of inhibitors identified for bacterial inorganic pyrophosphatases as starting points for new therapeutics.

Crystal structure of a hypothetical protein from Giardia lamblia. Corrigendum

The article by Beard et al. [(2022), Acta Cryst. F78, 59–65] is corrected.