issue contents
October 2023 issue

Cover illustration: An inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1 [Moorefield et al. (2023), Acta Cryst. F79, 257–266]. Inorganic pyrophosphate (PPi) is generated as an intermediate or byproduct of many fundamental metabolic pathways, including DNA/RNA synthesis. The crystal structure of a family I inorganic pyrophosphatase from Legionella pneumophila, a human opportunistic pathogen that is the major causative agent of Legionnaires' disease, has been determined.
research communications
Crystallization strategies for and structural analyses of all of the players in the faRel2/aTfaRel2 toxin–antitoxin system are reported.
J. Moorefield,
Y. Konuk,
J. O. Norman,
J. Abendroth,
T. E. Edwards,
D. D. Lorimer,
S. J. Mayclin,
B. L. Staker,
J. K. Craig,
K. F. Barett,
L. K. Barrett,
W. C. Van Voorhis,
P. J. Myler and
K. J. McLaughlin
The crystal structure of a soluble family I inorganic pyrophosphatase from L. pneumophila Philadelphia 1 was determined to a resolution of 2.0 Å. Inorganic pyrophosphatases play a central role in regulating cellular pyrophosphate concentration, which is important for many biosynthetic pathways. L. pneumophila Philadelphia 1 causes a rare but severe respiratory infection known as Legionnaires' disease.
methods communications
Guidance is provided on depositing raw diffraction data to support the results described in a macromolecular crystallography paper.
scientific commentaries