Crystallographic analysis of the Escherichia coli tRNA seleno-modification enzyme in complex with tRNA

tRNA 2-selenouridine synthase (SelU) produces a 5-substituted 2-selenouridine at the tRNA wobble position, which can fine-tune translation. Crystals of the SelU–tRNA complex were obtained and the initial structure was determined at 3.10 Å resolution.

The structure of His15 acetamide-modified hen egg-white lysozyme: a nice surprise from an old friend

Chemical modification of lysozyme at His15 using iodoacetamide was investigated structurally. Crystallization of the chemically modified enzyme using a protocol which yields tetragonal crystals gave orthorhombic crystals with similar unit-cell parameters.

Serendipitous high-resolution structure of Escherichia coli carbonic anhydrase 2

Contaminating proteins from the expression source may inadvertently crystallize instead of the target protein of interest. Two novel crystal forms of E. coli carbonic anhydrase 2, as well as a high-resolution (1.43 Å) structure of the enzyme, are reported.

Structural insights into the role of the prosegment binding loop in a papain-superfamily cysteine protease from Treponema denticola

TDE0362 is an essential factor for the pathogenicity of T. denticola in oral bacterial disease. This work provides insights into the regulatory mechanism of TDE0362 and identifies putative substrate-binding interfaces which could be used to guide further research towards inhibition of this enzyme.

CryoCrane: an open-source GUI for analyzing cryo-EM screening data sets

CryoCrane is a GUI which allows rapid analysis of cryo-EM screening data and supervision of data collections.