Crystal structure of thermally stable homodimeric cytochrome c′-β from Thermus thermophilus

Structural and thermal stability analyses of Thermus thermophilus cytochrome c-β (TTCP-β) are presented. It is shown that TTCP-β exhibits higher stability than moderately thermophilic homologue from Methylococcus capsulatus, although both proteins have quite similar folding, heme environment, and subunit composition.

Preliminary XFEL data from spontaneously grown endo-1,4-β-xylanase crystals from Hypocrea virens

Preliminary XFEL diffraction data for endo-1,4-β-xylanase from Hypocrea virens are presented.

Crystal structure of an extracellular superoxide dismutase from Onchocerca volvulus and implications for parasite-specific drug development

An extracellular Cu/Zn superoxide dismutase from Onchocerca volvulus, the causative agent of human onchocerciasis, was purified and crystallized and the structure was solved at 1.55 Å resolution. The solution structure of the dimeric protein was verified using small-angle X-ray scattering. Initial docking studies utilizing previously identified superoxide dismutase inhibitors indicate the potential for future drug development targeting structural features outside the active site.

In situ crystal data-collection and ligand-screening system at SPring-8

An in situ X-ray diffraction measurement system using a crystallization plate has been constructed at the SPring-8 protein crystallography beamline. Utilizing small-wedge measurements and incorporating a liquid dispenser to prepare protein–ligand complex crystals, this system will make ligand screening possible.