Identification, structure determination and analysis of Mycobacterium smegmatis acyl-carrier protein synthase (AcpS) crystallized serendipitously

This study reports the unintended crystallization and the structure determination of holo acyl-carrier protein synthase from Mycobacterium smegmatis.

Crystal structure of the catalytic domain of human RPTPH

The crystal structure of the catalytic domain of human RPTPH reveals information for the development of specific inhibitors.

Crystal structure determination of the halogenase CtcP from Streptomyces aureofaciens

The crystal structure of the selenomethionine-derivatized halogenase CtcP from Streptomyces aureofaciens, which functions in chlortetracycline biosynthesis, is reported at 2.7 Å resolution. The structure reveals a conserved monooxygenase domain and a unique C-terminal domain. Although FAD was not observed in the structure, the monooxygenase domain has a conserved FAD-binding pocket and active center.

Structural study of Legionella pneumophila effector DotY (Lpg0294), a component of the Dot/Icm type IV secretion system

DotY is a component of the inner membrane-embedded coupling complex T4CC that delivers effectors to the type IV secretion system translocation apparatus. DotY is a highly mobile component of this subcomplex. The crystal structure of DotY on its own was determined and its fold and the connectivity of its secondary-structure elements were established.

Serial crystallography with multi-stage merging of thousands of images

The effectiveness of clustering in merging data sets from large numbers of crystals in serial crystallography can be improved by combining multiple clustering techniques using unit-cell parameter-based clustering for very incomplete sets and switching to reflection-based clustering once the preliminary merging has increased the completeness.