AlphaFold and the future of structural biology

This editorial acknowledges the transformative impact of new machine-learning methods, such as the use of AlphaFold, but also makes the case for the continuing need for experimental structural biology.

Structure of a superoxide dismutase from a tardigrade: Ramazzottius varieornatus strain YOKOZUNA-1

Crystal structures of a wild-type superoxide dismutase from Ramazzottius varieornatus and its V87H mutant show that even though a histidine is placed at position 87, a nearby flexible loop can destabilize the coordination of His87 to the Cu atom.

Tetracycline-modifying enzyme SmTetX from Stenotrophomonas maltophilia

Stenotrophomonas maltophilia codes for a tetracycline-modifying FAD-dependent monooxygenase that shares an overall fold with the tetracycline destructase TetX and possesses a unique active site within this enzyme family.

Crystal structure of CmnB involved in the biosynthesis of the nonproteinogenic amino acid L-2,3-di­aminopropionic acid

The crystal structure of CmnB, which belongs to the PLP-dependent enzymes, was determined at 2.2 Å resolution. CmnB is involved in the formation of L-2,3-diaminopropionic acid in the biosynthesis of capreomycin.